Document Detail


Organized nerve culture. I. A technique to study the effect of M. leprae infection.
MedLine Citation:
PMID:  6995359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neonatal dorsal root ganglia were cultivated in vitro by the technique of Murray. Within a week bundles of organized nerve fibers containing proliferating Schwann cells in different phases of axon association and fibroblast cells destined to become peri- or endoneural cells were obtained, peri- or endoneural cells were obtained. Many of these nerve fibers were myelinated within 3-4 weeks. Such 1 or 2 week old cultures were inoculated with M. leprae, and bacilli were found in the cytoplasm of Schwann cells and fibroblasts, demonstrating that these cells are phagocytic in nature and that it is possible to infect them with M. leprae, Schwann cells, mostly in the free or early association phase, engulfed the bacilli, and this affected their further interaction with the axons and subsequent myelin synthesis.
Authors:
R Mukherjee; P R Mahadevan; N H Antia
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association     Volume:  48     ISSN:  0148-916X     ISO Abbreviation:  Int. J. Lepr. Other Mycobact. Dis.     Publication Date:  1980 Jun 
Date Detail:
Created Date:  1980-10-27     Completed Date:  1980-10-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8505819     Medline TA:  Int J Lepr Other Mycobact Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  183-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Culture Techniques
Fibroblasts / microbiology
Ganglia, Spinal / cytology,  microbiology*
Leprosy / immunology,  microbiology
Mice
Mycobacterium leprae / growth & development*,  immunology
Nerve Fibers / microbiology
Phagocytosis*
Schwann Cells / immunology*,  microbiology
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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