| Organization of nucleoli and nuclear bodies in osmotically stimulated supraoptic neurons of the rat. | |
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MedLine Citation:
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PMID: 1865004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study has analyzed variations in the number of nucleoli and nuclear bodies, as well as in their ultrastructural and cytochemical organization, after the osmotically induced activation of supraoptic nucleus (SON) neurons of the rat. The number of nucleoli and nuclear bodies and also the nucleolar size were determined on smear preparations of previously block-impregnated SON. The mean number of nucleoli per cell was 1.35 +/- 0.6 (mean +/- SDM) in control rats. No significant variations in this value were registered either in dehydrated or rehydrated rats. The mean nucleolar volume and the total nucleolar volume per cell showed a significant increase in dehydrated rats with respect to the controls, whereas these two parameters tended to return to control values in rats rehydrated after dehydration. The mean number of nuclear bodies per cell increased significantly from 0.56 +/- 0.50 (mean +/- SDM) in control rats to 1.54 +/- 1.1 after 6 days of dehydration. By electron microscopy, SON neurons displayed a reticulated nucleolar configuration. After the osmotically induced neuronal activation, there was an increase in the proportion of the total nucleolar area occupied by the granular component, and also a reduction in the mean fibrillar-center area. The most characteristic nucleolar features in rehydrated rats were the tendency for the granular component to be segregated and the occurrence of intranucleolar vacuoles. Ultrastructural cytochemistry with a specific silver method revealed a selective silver reaction on the coiled threads of the nuclear bodies--identified as "coiled bodies"--and on the nucleolar fibrillar components in all animal groups studied. Since nucleoli play a major role in ribosome biogenesis, a relationship between these nucleolar changes and the level of cellular activity of SON neurons is proposed. Furthermore, the response of nuclear "coiled bodies" to neuronal activation suggests their participation in the processing and transport of rRNA precursors. |
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Authors:
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M Lafarga; M A Andres; M T Berciano; E Maquiera |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of comparative neurology Volume: 308 ISSN: 0021-9967 ISO Abbreviation: J. Comp. Neurol. Publication Date: 1991 Jun |
Date Detail:
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Created Date: 1991-09-11 Completed Date: 1991-09-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 329-39 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology, Faculty of Medicine, Santander, Spain. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Nucleus / ultrastructure Osmosis Rats Rats, Inbred Strains / anatomy & histology* Supraoptic Nucleus / ultrastructure* |
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