Document Detail

Organization of mesosomes in fixed and unfixed cells.
MedLine Citation:
PMID:  821934     Owner:  NLM     Status:  MEDLINE    
After the addition of glutaraldehyde (GA) to cells incubated at 3 or 37 degrees C, mesosomes were observed with increasing frequencies in freeze fractures of cells. These increases were related to the kinetics with which GA cross-linked adjacent amino acids. Upon the addition of GA, mesosomes were first observed in the periphery of freeze-fractured cells usually attached to septal membranes. However, the time, while the septal attachment sites were maintained, the "bodies" of the mesosomes were observed to move toward the center of the cytoplasm. This centralization process was much more rapid at 37 than at 3 degrees C. It is hypothesized that upon fixation, or receipt of some physical insult, mesosome precursors found in undisturbed cells undergo a change in state that results in their visibility in freeze fractures.
M L Higgins; H C Tsien; L Daneo-Moore
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  127     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-11-01     Completed Date:  1976-11-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1519-23     Citation Subset:  IM    
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MeSH Terms
Aldehydes / pharmacology*
Enterococcus faecalis / ultrastructure*
Freeze Fracturing
Glutaral / pharmacology*
Models, Biological
Organoids / drug effects,  ultrastructure
Reg. No./Substance:
0/Aldehydes; 111-30-8/Glutaral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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