Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region.

Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region.

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MedLine Citation:	PMID: 6261224 Owner: NLM Status: MEDLINE
Abstract/OtherAbstract:	The product of the lexA gene of Escherichia coli has been shown to regulate expression of the several cellular functions (SOS functions) induced by treatments which abruptly inhibit DNA synthesis. We have cloned and mapped the lexA gene on a small segment of approximately 600 base pairs. The lexA promotor was located by transcription R-loop analysis, and the lexA product of 22,000 daltons was identified by protein synthesis in vitro. An unknown gene was found which directed the synthesis of a protein of 35,000 daltons in a region downstream from the lexA gene. Nucleotide sequence of the regulatory region of the lexA gene was determined. The sequence contained inverted repeats homologous to that of the recA regulatory region. These inverted repeats may be recognized by the lexA protein, because the protein is considered to repress both the genes as a common repressor.

Authors:	T Miki; Y Ebina; F Kishi; A Nakazawa
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Publication Detail:	Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Detail:	Title: Nucleic acids research Volume: 9 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1981 Feb
Date Detail:	Created Date: 1981-06-23 Completed Date: 1981-06-23 Revised Date: 2009-11-18
Medline Journal Info:	Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND
Other Details:	Languages: eng Pagination: 529-43 Citation Subset: IM
Data Bank Information
Bank Name/Acc. No.:	GENBANK/J01643; V00299; V00300
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MeSH Terms
Descriptor/Qualifier:	Bacterial Proteins / biosynthesis, genetics* Base Sequence Chromosome Mapping DNA Restriction Enzymes Escherichia coli / genetics* Genes Operon Plasmids Repressor Proteins / genetics Transcription, Genetic
Chemical
Reg. No./Substance:	0/Bacterial Proteins; 0/Repressor Proteins; EC 3.1.21.-/DNA Restriction Enzymes
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962	Article Information Download PDF Print publication date: Day: 11 Month: 2 Year: 1981 Volume: 9 Issue: 3 First Page: 529 Last Page: 543 ID: 327220 PubMed Id: 6261224