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Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region.
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MedLine Citation:
PMID:  6261224     Owner:  NLM     Status:  MEDLINE    
The product of the lexA gene of Escherichia coli has been shown to regulate expression of the several cellular functions (SOS functions) induced by treatments which abruptly inhibit DNA synthesis. We have cloned and mapped the lexA gene on a small segment of approximately 600 base pairs. The lexA promotor was located by transcription R-loop analysis, and the lexA product of 22,000 daltons was identified by protein synthesis in vitro. An unknown gene was found which directed the synthesis of a protein of 35,000 daltons in a region downstream from the lexA gene. Nucleotide sequence of the regulatory region of the lexA gene was determined. The sequence contained inverted repeats homologous to that of the recA regulatory region. These inverted repeats may be recognized by the lexA protein, because the protein is considered to repress both the genes as a common repressor.
T Miki; Y Ebina; F Kishi; A Nakazawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  9     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1981 Feb 
Date Detail:
Created Date:  1981-06-23     Completed Date:  1981-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  529-43     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J01643;  V00299;  V00300
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MeSH Terms
Bacterial Proteins / biosynthesis,  genetics*
Base Sequence
Chromosome Mapping
DNA Restriction Enzymes
Escherichia coli / genetics*
Repressor Proteins / genetics
Transcription, Genetic
Reg. No./Substance:
0/Bacterial Proteins; 0/Repressor Proteins; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 2 Year: 1981
Volume: 9 Issue: 3
First Page: 529 Last Page: 543
ID: 327220
PubMed Id: 6261224

Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region.
T Miki
Y Ebina
F Kishi
A Nakazawa

Article Categories:
  • Research Article

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