Document Detail


Organization of the gene encoding the mouse T-cell-specific serine proteinase "granzyme A".
MedLine Citation:
PMID:  1639378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mouse serine protease granzyme A is a member of a closely related family of T-cell-associated proteolytic enzymes, designated granzymes A-G. Previous studies have indicated that granzymes A and B are involved in various T-cell-mediated processes. Here we report the genomic organization of the granzyme A gene. We have cloned a 15-kb DNA fragment from a genomic library of a cloned CD8+ T-cell line and sequenced the exon-intron boundaries. The gene consists of five exons, and its genomic organization is very similar to that described for granzymes B, C, and F. In addition, we have sequenced 1.4 kb of the 5'-region and 1.1 kb of the 3'-region flanking the granzyme A gene. Putative promoter and enhancer elements were identified by sequence comparison with known consensus sequences. Some of these regulatory elements seem to be associated exclusively with granzyme A, whereas others are shared by members of the granzyme family.
Authors:
K Ebnet; M D Kramer; M M Simon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  13     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-28     Completed Date:  1992-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  502-8     Citation Subset:  IM    
Affiliation:
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M74211;  M74212;  M74213;  M74214;  M74215;  S41693;  X60310;  X60311;  X62542;  X62543
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
DNA / genetics
Exons
Granzymes
Introns
Mice
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Serine Endopeptidases / genetics*
T-Lymphocytes / enzymology*
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 3.4.21.-/Granzymes; EC 3.4.21.-/Serine Endopeptidases

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