Document Detail


Organization of the double-stranded RNA-activated protein kinase DAI and virus-associated VA RNAI in adenovirus-2-infected HeLa cells.
MedLine Citation:
PMID:  7903669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined the cellular distribution of the double-stranded RNA-activated protein kinase DAI in adenovirus 2 (Ad2)-infected and uninfected HeLa cells. In uninfected cells DAI was found to be concentrated in the cytoplasm. In addition, DAI was localized in the nucleoli and diffusely distributed throughout the nucleoplasm. Cells treated with alpha-interferon displayed a similar pattern of distribution for DAI. When RNA polymerase I activity was inhibited by the drug actinomycin D, nucleoli segregated and DAI was found to colocalize with the dense fibrillar region of the nucleoli. During mitosis, the distribution of DAI paralleled that of rRNA. In adenovirus-infected cells the localization of DAI was similar to that in uninfected interphase cells. VA RNAI was detected in Ad2-infected cells by 10-14 hours post-infection as fine dots in the nucleoplasm. By 18-24 hours post-infection, VA RNAI appeared in bigger and more abundant dots in the nucleoplasm and the cytoplasm was intensively labeled. Transient expression of the VA RNAI gene in uninfected cells resulted in a similar localization of the RNA. Our results are consistent with a role for DAI and VA RNAI in protein synthesis and suggest that DAI may play an early role in ribosome biogenesis in the nucleolus in addition to its cytoplasmic role in translation.
Authors:
L F Jiménez-García; S R Green; M B Mathews; D L Spector
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  106 ( Pt 1)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1994-01-31     Completed Date:  1994-01-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  11-22     Citation Subset:  IM    
Affiliation:
Cold Spring Harbor Laboratory, New York 11724.
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MeSH Terms
Descriptor/Qualifier:
Adenoviruses, Human / genetics*
Cell Compartmentation
Cell Nucleolus / enzymology*
Cytoplasm / enzymology
Dactinomycin / pharmacology
Hela Cells / enzymology*,  microbiology
Humans
In Situ Hybridization
Interphase
Microscopy, Fluorescence
Protein-Serine-Threonine Kinases / metabolism*
RNA, Double-Stranded / physiology*
RNA, Viral / analysis*
eIF-2 Kinase
Grant Support
ID/Acronym/Agency:
5P30 CA45508-03/CA/NCI NIH HHS; CA 13106/CA/NCI NIH HHS; GM42694/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Double-Stranded; 0/RNA, Viral; 50-76-0/Dactinomycin; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/eIF-2 Kinase

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