Document Detail


Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA.
MedLine Citation:
PMID:  2990828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human Y chromosome contains a group of repeated DNA elements, identified as 3.4-kilobase pair (kb) fragments in Hae III digests of male genomic DNA, which contain both Y-specific and non-Y-specific sequences. We have used these 3.4-kb Hae III Y fragments to explore the organizational properties and chromosomal distribution of the autosomal homologs of the non-Y-specific (NYS) 3.4-kb Hae III Y elements. Three distinct organizations, termed domains, have been identified and shown to have major concentrations on separate chromosomes. We have established that domain K is located on chromosome 15 and domain D on chromosome 16 and suggested that domain R is on chromosome 1. Our findings suggest that each domain is composed of a tandemly arrayed cluster of a regularly repeating unit containing two sets of repeated sequences: one that is homologous to the NYS 3.4-kb Hae III Y sequences and one that does not cross-react with the 3.4-kb Hae III Y repeats. Thus, these autosomal repeated DNA domains, like their Y chromosome counterparts, consist of a complex mixture of repeated DNA elements interspersed among each other in ways that lead to defined periodicities. Although each of the three identified autosomal domains cross-reacts with 3.4-kb Hae III Y fragments purified from genomic DNA, the length periodicities and sequence content of the autosomal domains are chromosome specific. The organizational properties and chromosomal distribution of these NYS 3.4-kb Hae III homologs seem inconsistent with stochastic mechanisms of sequence diffusion between chromosomes.
Authors:
R D Burk; P Szabo; S O'Brien; W G Nash; L Yu; K D Smith
Related Documents :
15317218 - Molecular genetic analysis of tpo and cd4 loci among two endogamous ethnic groups of ma...
10987648 - Isolation of cag/ctg repeats from within the chromosome 2p21-p24 locus for autosomal do...
8422498 - Characterization of four vntr loci on human chromosome 6.
12176908 - Identification of a novel ncf-1 (p47-phox) pseudogene not containing the signature gt d...
6971608 - Partial distal 12q trisomy.
8950408 - No evidence for linkage of chromosome 22 markers to schizophrenia in southern african b...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  92     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1985  
Date Detail:
Created Date:  1985-09-16     Completed Date:  1985-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  225-33     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Nucleus / analysis
Cricetinae
Cricetulus
DNA / isolation & purification*
DNA Restriction Enzymes
Female
Humans
Hybrid Cells / cytology
Male
Molecular Weight
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
Sex Factors
Y Chromosome / analysis*
Grant Support
ID/Acronym/Agency:
GM 07471/GM/NIGMS NIH HHS; HD 17161/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A presumptive role for leukotrienes in obstructive airways diseases.
Next Document:  Flow cytometric evaluation of cell dispersion from human head and neck tumors.