Document Detail

Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA.
MedLine Citation:
PMID:  2990828     Owner:  NLM     Status:  MEDLINE    
The human Y chromosome contains a group of repeated DNA elements, identified as 3.4-kilobase pair (kb) fragments in Hae III digests of male genomic DNA, which contain both Y-specific and non-Y-specific sequences. We have used these 3.4-kb Hae III Y fragments to explore the organizational properties and chromosomal distribution of the autosomal homologs of the non-Y-specific (NYS) 3.4-kb Hae III Y elements. Three distinct organizations, termed domains, have been identified and shown to have major concentrations on separate chromosomes. We have established that domain K is located on chromosome 15 and domain D on chromosome 16 and suggested that domain R is on chromosome 1. Our findings suggest that each domain is composed of a tandemly arrayed cluster of a regularly repeating unit containing two sets of repeated sequences: one that is homologous to the NYS 3.4-kb Hae III Y sequences and one that does not cross-react with the 3.4-kb Hae III Y repeats. Thus, these autosomal repeated DNA domains, like their Y chromosome counterparts, consist of a complex mixture of repeated DNA elements interspersed among each other in ways that lead to defined periodicities. Although each of the three identified autosomal domains cross-reacts with 3.4-kb Hae III Y fragments purified from genomic DNA, the length periodicities and sequence content of the autosomal domains are chromosome specific. The organizational properties and chromosomal distribution of these NYS 3.4-kb Hae III homologs seem inconsistent with stochastic mechanisms of sequence diffusion between chromosomes.
R D Burk; P Szabo; S O'Brien; W G Nash; L Yu; K D Smith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  92     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1985  
Date Detail:
Created Date:  1985-09-16     Completed Date:  1985-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  225-33     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Nucleus / analysis
DNA / isolation & purification*
DNA Restriction Enzymes
Hybrid Cells / cytology
Molecular Weight
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
Sex Factors
Y Chromosome / analysis*
Grant Support
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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