Document Detail


Organic phase synthesis of ethyl oleate using lipases produced by solid-state fermentation.
MedLine Citation:
PMID:  18392560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper reports a study of the enzymatic esterification of oleic acid and ethanol. The reaction was catalyzed by lipases produced by solid-state fermentation with Rhizopus sp. Olive oil and perlite were used as an inducer and inert support, respectively. Synthesis of ethyl oleate was carried out in a 10-mL batch reactor with magnetic stirring. The effects of substrate ratios, biocatalyst concentration, and temperature on the reaction rate and conversion efficiency were evaluated. The highest reaction rate (1.64 mmol/L min) was reached with an oleic acid/ethanol mol ratio of 1:5 (oleic acid 50 mM:ethanol 250 mM) and 1 g of biocatalyst. Conversions approaching 100% were obtained after 60 min of reaction at 45 degrees C with n-hexane as a solvent. The initial reaction rate increased proportionally with respect to biocatalyst concentration, which suggests that the reaction rate was not controlled by mass transfer. The biocatalyst retained more than 80% of its catalytic activity after 7 months of storage at 4 degrees C. The results demonstrate that the biocatalyst produced by Rhizopus sp. in solid-state fermentation can be successfully used for ethyl oleate synthesis over short reaction periods under conditions when ethanol is in excess.
Authors:
Antonio Martínez-Ruiz; Hugo Sergio García; Gerardo Saucedo-Castañeda; Ernesto Favela-Torres
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-05
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  151     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-31     Completed Date:  2008-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  393-401     Citation Subset:  IM    
Affiliation:
Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Col Vicentina, Mexico City, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Enzyme Stability
Esters / chemical synthesis
Ethanol / metabolism
Fermentation
Lipase / metabolism*
Oleic Acid / metabolism
Oleic Acids / biosynthesis*
Rhizopus / enzymology
Temperature
Chemical
Reg. No./Substance:
0/Esters; 0/Oleic Acids; 111-62-6/ethyl oleate; 112-80-1/Oleic Acid; 64-17-5/Ethanol; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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