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Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response.
MedLine Citation:
PMID:  22910366     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A key molecule of sensing machineries essential for survival upon hypo-osmotic shock is the mechanosensitive channel. The bacterial mechanosensitive channel MscS functions directly for this purpose by releasing cytoplasmic solutes out of the cell, whereas plant MscS homologues are found to function in chloroplast organization. Here we show that the fission yeast MscS homologues, designated Msy1 and Msy2, participate in the hypo-osmotic shock response by a mechanism different from that operated by the bacterial MscS. Upon hypo-osmotic shock, msy2(-) and msy1(-) msy2(-) cells display greater cell swelling than wild-type cells and undergo cell death. Cell swelling precedes an intracellular Ca(2+) increase, which was greater in msy1(-) and msy1(-) msy2(-) cells than in wild-type cells. Fluorescent microscopy showed that Msy1 and Msy2 localize mainly to the endoplasmic reticulum. These observations suggest that organellar Msy1 and Msy2 regulate intracellular Ca(2+) and cell volume for survival upon hypo-osmotic shock.
Authors:
Yoshitaka Nakayama; Kenjiro Yoshimura; Hidetoshi Iida
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature communications     Volume:  3     ISSN:  2041-1723     ISO Abbreviation:  Nat Commun     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101528555     Medline TA:  Nat Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  1020     Citation Subset:  IM    
Affiliation:
Department of Biology, Tokyo Gakugei University, 4-1-1 Nukui Kita-machi, Koganei-shi, Tokyo 184-8501, Japan.
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