Document Detail

Organellar and cytosolic localization of four phosphoribosyl diphosphate synthase isozymes in spinach.
MedLine Citation:
PMID:  9952445     Owner:  NLM     Status:  MEDLINE    
Four cDNAs encoding phosphoribosyl diphosphate (PRPP) synthase were isolated from a spinach (Spinacia oleracea) cDNA library by complementation of an Escherichia coli Deltaprs mutation. The four gene products produced PRPP in vitro from ATP and ribose-5-phosphate. Two of the enzymes (isozymes 1 and 2) required inorganic phosphate for activity, whereas the others were phosphate independent. PRPP synthase isozymes 2 and 3 contained 76 and 87 amino acid extensions, respectively, at their N-terminal ends in comparison with other PRPP synthases. Isozyme 2 was synthesized in vitro and shown to be imported and processed by pea (Pisum sativum) chloroplasts. Amino acid sequence analysis indicated that isozyme 3 may be transported to mitochondria and that isozyme 4 may be located in the cytosol. The deduced amino acid sequences of isozymes 1 and 2 and isozymes 3 and 4 were 88% and 75% identical, respectively. In contrast, the amino acid identities of PRPP synthase isozyme 1 or 2 with 3 or 4 was modest (22%-25%), but the sequence motifs for binding of PRPP and divalent cation-nucleotide were identified in all four sequences. The results indicate that PRPP synthase isozymes 3 and 4 belong to a new class of PRPP synthases that may be specific to plants.
B N Krath; B Hove-Jensen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  119     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-15     Completed Date:  1999-04-15     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  497-506     Citation Subset:  IM    
Center for Enzyme Research, Institute of Molecular Biology, University of Copenhagen, 83H Solvgade, DK-1307 Copenhagen K, Denmark.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ006940;  AJ006941;  AJ006942;  AJ006943
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MeSH Terms
Amino Acid Sequence
Base Sequence
Chloroplasts / enzymology
Cloning, Molecular
Cytosol / enzymology
DNA, Complementary / genetics,  isolation & purification
DNA, Plant / genetics,  isolation & purification
Escherichia coli / genetics
Genetic Complementation Test
Isoenzymes / genetics,  metabolism*
Mitochondria / enzymology
Molecular Sequence Data
Organelles / enzymology
Peas / enzymology
RNA, Messenger / genetics
RNA, Plant / genetics
Ribose-Phosphate Pyrophosphokinase / genetics,  metabolism*
Sequence Homology, Amino Acid
Spinacia oleracea / enzymology*,  genetics
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Isoenzymes; 0/RNA, Messenger; 0/RNA, Plant; EC Pyrophosphokinase

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