Document Detail

Organ specificity of the structural organization and fine distribution of lymphatic capillary networks: histochemical study.
MedLine Citation:
PMID:  10668209     Owner:  NLM     Status:  MEDLINE    
Histochemical studies of the microcirculatory system were reviewed with regard to the organ specificity of the structural organization and fine distribution of the lymphatic capillary network. The lymphatics and blood vessels are characterized by an enzyme-histochemical method using 5'-nucleotidase (5'-Nase), alkaline phosphatase (ALPase) and/or diaminopeptidase (DAPase) staining in addition to an immunohistochemical method. The 5'-Nase-positive lymphatic vessels can be distinguished histochemically from arterial and venous vessels based on ALPase and DAPase activity, respectively. The specificity and localization of the enzyme reactions were confirmed by comparative histochemical studies of the same specimen with light microscopy and scanning or transmission electron microscopy. These histochemical methods are discussed in relation to their ability to demonstrate the organ specificity of vascular networks under normal and pathological conditions.
S Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Histology and histopathology     Volume:  15     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-29     Completed Date:  2000-02-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  185-97     Citation Subset:  IM    
Department of Anatomy, Oita Medical University, Japan.
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MeSH Terms
Histocytochemistry / methods*
Immunohistochemistry / methods
Lymphatic System / cytology*,  ultrastructure
Microscopy / methods
Microscopy, Electron / methods
Organ Specificity

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