Document Detail

Ordered association of tobacco mosaic virus in the presence of divalent metal ions.
MedLine Citation:
PMID:  11374586     Owner:  NLM     Status:  MEDLINE    
The formation of ordered aggregates of tobacco mosaic virus (TMV) in the presence of divalent metal ions has been studied in concentrated (1-25 mg/ml) solutions of the virus. The divalent metal cations Cd2+, Zn2+, Pb2+, Cu2+, and Ni2+ have been found to promote TMV precipitation from solution at a critical concentration Ccrit, which for a given metal depends on the pH and the ionic strength of the solution, but is largely independent of the virus concentration. The TMV precipitate behaves as a nematic liquid crystal and on drying at a glass surface produces highly ordered, optically birefringent films. However, precipitation is not observed with alkali-earth metals such as Ca2+ and Mg2+. The experimental data suggest that, apart from two 'internal' metal-binding sites in each TMV subunit, the virus contains metal-binding sites of a lower affinity which promote cross-linking of TMV rods via metal bridges. The latter seem to be responsible for the precipitation of TMV in the presence of divalent cations at neutral pH. We propose that the metal-induced cross-linking may be the predominant mechanism to account for the limited solubility of a variety of proteins in solution containing metal cations with valence 2 and higher.
A Nedoluzhko; T Douglas
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of inorganic biochemistry     Volume:  84     ISSN:  0162-0134     ISO Abbreviation:  J. Inorg. Biochem.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-25     Completed Date:  2001-12-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7905788     Medline TA:  J Inorg Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  233-40     Citation Subset:  IM    
Department of Chemistry, Temple University, Philadelphia, PA 19122, USA.
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MeSH Terms
Binding Sites
Cations, Divalent
Chemical Precipitation
Hydrogen-Ion Concentration
Metals, Heavy / chemistry*
Microscopy, Electron
Tobacco Mosaic Virus / chemistry*
Reg. No./Substance:
0/Cations, Divalent; 0/Metals, Heavy

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