| Oral immunization with Porphyromonas gingivalis outer membrane protein and CpGoligodeoxynucleotides elicits T helper 1 and 2 cytokines for enhanced protective immunity. | |
| | |
MedLine Citation:
|
PMID: 20502628 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aim of this study was to evaluate the efficacy of an oral vaccine containing the 40-kDa outer membrane protein of Porphyromonas gingivalis (40K OMP) and synthetic oligodeoxynucleotides containing unmethylated CpG dinucleotides (CpG ODN) to control oral infection by P. gingivalis. [run on]40K-OMP40K-OMP40K-OMPOral immunization with 40K-OMP plus CpG ODN induced significant 40K-OMP-specific serum IgG, IgA and saliva IgA antibody responses. The 40K-OMP-specific CD4(+) T cells induced by oral 40K-OMP plus CpG ODN produced both Th1 (IFN-gamma) and Th2 (IL-4) cytokines. Furthermore, increased frequencies of CD11c(+)B220(+) DCs and CD11c(+)CD11b(+) DCs with up-regulated expression of CD80, CD86, CD40 and MHC II molecules were noted in spleen, Peyer's patches and cervical lymph nodes. Immunized mice were then infected orally with P. gingivalis to determine whether the immune responses induced by oral 40K-OMP plus CpG ODN were capable of suppressing bone resorption caused by P. gingivalis infection. Mice given 40K-OMP plus CpG ODN showed significantly reduced bone loss associated with oral infection by P. gingivalis.Thus, oral administration of 40K-OMP together with CpG ODN induces Th1- and Th2-type cells, which provide help for protective immunity against P. gingivalis infection. This may be an important tool for prevention of chronic periodontitis. |
| | |
Authors:
|
Chenlu Liu; Tomomi Hashizume; Tomoko Kurita-Ochiai; Kohtaro Fujihashi; Masafumi Yamamoto |
Related Documents
:
|
16229898 - The in vitro effects of cpg oligodeoxynucleotides on the expression of cytokine genes i... 10496308 - Modulation of macrophage and b cell function by glycosaminoglycans. 10603398 - Interleukin-12 as an adjuvant promotes immunoglobulin g and type 1 cytokine recall resp... 10976538 - The role of interleukin-12 and interferon-gamma in gvhd and gvl. 18087678 - Inflammation, inflammatory cells and angiogenesis: decisions and indecisions. 11991888 - Diesel exhaust particles enhance lung injury related to bacterial endotoxin through exp... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Molecular oral microbiology Volume: 25 ISSN: 2041-1014 ISO Abbreviation: Mol Oral Microbiol Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-11 Completed Date: 2010-09-22 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 101524770 Medline TA: Mol Oral Microbiol Country: Denmark |
Other Details:
|
Languages: eng Pagination: 178-89 Citation Subset: D |
Affiliation:
|
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Administration, Oral Alveolar Bone Loss / immunology, prevention & control* Animals Antibodies, Bacterial / blood Bacterial Outer Membrane Proteins / immunology* Bacterial Vaccines / administration & dosage, chemistry* Bacteroidaceae Infections / immunology, prevention & control* Chronic Periodontitis / immunology, prevention & control* CpG Islands / immunology* Dendritic Cells / physiology Female Interferon-gamma / biosynthesis Interleukin-4 / biosynthesis Mice Mice, Inbred BALB C Oligodeoxyribonucleotides / immunology* Porphyromonas gingivalis / chemistry, immunology* Quorum Sensing / genetics Th1 Cells / metabolism Th2 Cells / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
AG 025873/AG/NIA NIH HHS; DE12242/DE/NIDCR NIH HHS; R01 AG025873-04/AG/NIA NIH HHS; R01 DE012242-13/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Vaccines; 0/Oligodeoxyribonucleotides; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Facial Image Classification of Mouse Embryos for the Animal Model Study of Fetal Alcohol Syndrome.
Next Document: Model-Based Background Correction (MBCB): R Methods and GUI for Illumina Bead-array Data.