Document Detail

Oral glutamine attenuates surgical manipulation-induced alterations in the intestinal brush border membrane.
MedLine Citation:
PMID:  14572786     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Our earlier work has shown that surgical manipulation of the intestine results in oxidative stress and mucosal damage along with alterations in the brush border membrane (BBM). Glutamine feeding is known to offer protection against damage to mucosa under various conditions and this study looked at the effect of oral supplementation of glutamine or glutamic acid in the intestinal BBM alterations after surgical manipulation. MATERIALS AND METHODS: Control and rats pretreated for 7 days with 2% glutamine or glutamic acid or isonitrogenous amino acids, glycine, or alanine were subjected to surgical manipulation of the intestine. BBMs were isolated from the intestine and functional and structural alterations to these membranes were assessed and compared. RESULTS: Surgical manipulation resulted in oxidative stress in the enterocyte BBM and these changes included a decrease in alkaline phosphatase activity and alpha-tocopherol content along with an increase in lipid peroxidation parameters. A decrease in glucose transport by the isolated BBM vesicles suggested functional impairment. Surgical manipulation also resulted in phospholipid degradation possibly mediated by PLA(2) and membrane protease activation. Glutamine or glutamic acid supplementation prevented these changes but not by glycine or alanine. CONCLUSION: This study suggests that oral glutamine or glutamic acid supplementation prior to surgery can offer protection to the intestine and this might prevent postsurgical complications.
Ramamoorthy Prabhu; Simmy Thomas; Kunisserry A Balasubramanian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of surgical research     Volume:  115     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-23     Completed Date:  2003-11-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  148-56     Citation Subset:  IM    
The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College, Vellore 632004, India.
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MeSH Terms
Alanine / administration & dosage
Alkaline Phosphatase / metabolism
Biological Transport
Glucose / metabolism
Glutamic Acid / administration & dosage
Glutamine / administration & dosage*
Glutathione / analysis
Glycine / administration & dosage
Intestines / surgery*,  ultrastructure*
Lipid Peroxidation
Microvilli / chemistry,  metabolism,  ultrastructure*
Oxidative Stress
Phospholipids / metabolism
Rats, Wistar
alpha-Tocopherol / analysis
Reg. No./Substance:
0/Phospholipids; 50-99-7/Glucose; 56-40-6/Glycine; 56-41-7/Alanine; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; 59-02-9/alpha-Tocopherol; 70-18-8/Glutathione; EC Phosphatase

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