Document Detail

Oral cow milk challenge abolishes antigen-specific interferon-gamma production in the peripheral blood of children with atopic dermatitis and cow milk allergy.
MedLine Citation:
PMID:  9088654     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: A major feature of atopic dermatitis (AD) is the propensity to generate IgE to environmental antigens. Despite extensive information on IgE dysregulation in AD, the nature of immune responses to ingested allergens is poorly characterized. OBJECTIVE: To determine the clinical and immunological responses to food in AD patients. METHODS: To characterize the type and timing of clinical reactions to oral cow milk, 83 AD patients aged 2 to 60 months were subjected to double-blind placebo-controlled food challenge (DBPCFC). IFN-gamma and IL-4 production by their peripheral blood mononuclear cells (PBMC) was determined before and after DBPCFC. RESULTS: Of 50 patients positive to DBPCFC. 46% manifested exanthematous-type immediate-onset reactions and 54% eczematous-type late-onset reactions. In either group, the production of IL-4 and IFN-gamma by Concanavalin A-stimulated PBMC was comparable before and after DBPCFC. For immediate-reacting patients, the median IFN-gamma production by milk-stimulated PBMC was 11.5 (4.2-17.2) pg/ml as against 2.3 (0.2-5.7) pg/ml by unstimulated PBMC, P = 0.0008 before DBPCFC, and 4.6 (2.8-10.3) pg/ml. vs 4.2 (1.7-9.0) pg/mL, p = 0.40, correspondingly after DBPCFC. CONCLUSION: Before DBPCFC, immediate-reacting but not late-reacting patients were found to be capable of allergen-specific IFN-gamma production in vitro, indicating the heterogeneity in AD patients. After DBPCFC, the IFN-gamma generation abolished, indicating the effect of oral allergen exposure on IFN-gamma producing responses of AD patients.
Y Sütas; M Hurme; E Isolauri
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  27     ISSN:  0954-7894     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-10     Completed Date:  1997-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  277-83     Citation Subset:  IM    
Department of Pediatrics, Universities of Tampere and Turku, Finland.
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MeSH Terms
Child, Preschool
Dermatitis, Atopic / blood,  immunology*,  physiopathology
Immunoglobulin E / blood
Interferon-gamma / blood*
Milk / adverse effects*
Milk Hypersensitivity / immunology*
Reg. No./Substance:
37341-29-0/Immunoglobulin E; 82115-62-6/Interferon-gamma

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