Document Detail


Oral cancer cells differ from normal oral epithelial cells in tissue like organization and in response to lycopene treatment: an organotypic cell culture study.
MedLine Citation:
PMID:  15087273     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We established distinctive monolayer and organotypic cell culture techniques to assess possible differences in cross-talk and spatial and structural organization of oral cancer cells compared with normal oral cells and also to evaluate possible differential responses of the cells to carotenoids. In monolayers, we investigated the effect of lycopene on the proliferation of an established oral cancer cell line, KB-1, and compared it with a primary cell line obtained from normal oral mucosa. Lycopene exerted a significant inhibitory effect on KB-1 cell proliferation inducing a dose-dependent downregulation of proliferating cell nuclear antigen (PCNA) associated with upregulation of connexin-43 (Cx-43) expression, whereas in the normal oral mucosal cells lycopene did not affect either PCNA expression, which was very low, or the expression of Cx-43, which was basically very high. Lycopene significantly inhibited the formation of colonies induced by the carcinogen 3-methylcholanthrene (MCA) on normal oral cells and almost completely abrogated the hyperplastic effect induced by MCA. KB-1 cells and normal oral epithelial cells in the organotypic cell culture method differed in their stratification and intercellular adhesion patterns as well as in the expression profile of cytokeratins, vimentin, and Cx-43. Lycopene induced Cx-43 expression in KB-1 cells grown by the organotypic raft method, similar to KB-1 cells grown as monolayers. We conclude that lycopene is a promising chemopreventive, pro-differentiating, and anticarcinogenic agent. No adverse effects of lycopene were detected in normal cells cultured in either monolayer or organotypic systems.
Authors:
Orly Livny; Ilana Kaplan; Ram Reifen; Sylvie Polak-Charcon; Zecharia Madar; Betty Schwartz
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Nutrition and cancer     Volume:  47     ISSN:  0163-5581     ISO Abbreviation:  Nutr Cancer     Publication Date:  2003  
Date Detail:
Created Date:  2004-04-16     Completed Date:  2004-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  195-209     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, 76100, Israel.
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MeSH Terms
Descriptor/Qualifier:
Anticarcinogenic Agents / pharmacology*
Blotting, Western
Carotenoids / pharmacology*
Cell Communication / drug effects
Cell Division / drug effects
Connexin 43 / genetics,  metabolism
Dose-Response Relationship, Drug
Epithelial Cells / cytology,  drug effects*
Gap Junctions / drug effects
Humans
Immunohistochemistry
Mouth Neoplasms / genetics,  metabolism,  ultrastructure*
Proliferating Cell Nuclear Antigen / analysis
Tumor Cells, Cultured / cytology,  drug effects*
Up-Regulation
Chemical
Reg. No./Substance:
0/Anticarcinogenic Agents; 0/Connexin 43; 0/Proliferating Cell Nuclear Antigen; 36-88-4/Carotenoids; 502-65-8/lycopene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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