| Oral brush biopsy analysis by matrix assisted laser desorption/ionisation-time of flight mass spectrometry profiling - A pilot study. | |
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MedLine Citation:
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PMID: 21354855 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Oral squamous cell carcinomas (OSCCs) often present as advanced tumours requiring aggressive local and regional therapy and result in significant functional impairment. The objective is to develop pre-symptomatic screening detection of OSCC by a brush biopsy method which is less invasive than the conventional biopsy for histology. Given the molecular heterogeneity of oral cancer, it is unlikely that even a panel of tumour markers would provide accurate diagnosis. Therefore, approaches such as the matrix-assisted-laser-desorption/ionisation-time-of-flight-mass-spectrometry (MALDI-TOF-MS) allow several biomarkers or peptide profile patterns to be simultaneously assessed. Brush biopsies from 27 patients with histology-proven OSCCs plus 40 biopsies from 10 healthy controls were collected. MALDI-TOF-MS profiling was performed and additional statistical analysis of the data was used to classify the disease status according to the biological behaviour of the lesion. For classification a support vector machine algorithm was trained using spectra of brush biopsy samples to distinguish healthy control patients from patients with histology-proven OSCC. MALDI-TOF-MS was able to distinguish between healthy patients and OSCC patients with a sensitivity of 100% and specificity of 93%. In summary, MALDI-TOF-MS in combination with sophisticated bioinformatic methods can distinguish OSCC patients from non-cancer controls with excellent sensitivity and specificity. Further improvement and validation of this approach is necessary to determine its feasibility to assist the pre-symptomatic detection of head and neck cancer screening in routine daily practice. |
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Authors:
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Torsten W Remmerbach; Katja Maurer; Sebastian Janke; Wolfgang Schellenberger; Klaus Eschrich; Julia Bertolini; Herbert Hofmann; Stefan Rupf |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-25 |
Journal Detail:
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Title: Oral oncology Volume: - ISSN: 1368-8375 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9709118 Medline TA: Oral Oncol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Oral & Maxillofacial and Facial Plastic Surgery, University of Leipzig, Leipzig, Germany; Griffith Health Institute, Gold Coast campus, Griffith University, QLD 4222, Australia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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