Document Detail

Oral administration of KW-5092, a novel gastroprokinetic agent with acetylcholinesterase inhibitory and acetylcholine release enhancing activities, causes a dose-dependent increase in the blood acetylcholine content of beagle dogs.
MedLine Citation:
PMID:  9143009     Owner:  NLM     Status:  MEDLINE    
Acetylcholine (ACh) was detected in the blood and plasma of beagle dogs using a specific, sensitive radioimmunoassay. The mean basal ACh contents in the blood and plasma of beagle dogs were 451 +/- 65 and 83.5 +/- 12.3 pg/ml (+/- SEM, n = 7), respectively, and were lower than the contents in humans reported previously by our laboratory. Oral administration of KW-5092 (10-30 mg/kg), a gastroprokinetic agent with acetylcholinesterase (AChE) inhibitory and ACh release enhancing activities, caused a dose-dependent increase in the ACh content of both the blood and plasma, as well as several behavioral side effects due to peripheral cholinergic stimulation. The size of the increase in the plasma ACh content at each dose of KW-5092 was greater than that in the blood, indicating that KW-5092 caused the increase in the blood ACh content through elevation of the plasma ACh content, by inhibition of AChE and facilitation of ACh release. These results demonstrate that the blood ACh of beagle dogs is present mainly in the blood cells and to a lesser degree in the plasma, and that KW-5092 increased the blood ACh content mainly by increasing the plasma ACh concentration.
S Yamada; T Fujii; K Kawashima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroscience letters     Volume:  225     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-07-24     Completed Date:  1997-07-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  25-8     Citation Subset:  IM    
Department of Pharmacology, Kyoritsu College of Pharmacy, Tokyo, Japan.
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MeSH Terms
Acetylcholine / blood*
Administration, Oral
Cholinesterase Inhibitors / pharmacology*
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Gastrointestinal Agents / pharmacology*
Gastrointestinal Motility / drug effects*
Imidazoles / pharmacology*
Nitriles / pharmacology*
Reg. No./Substance:
0/Cholinesterase Inhibitors; 0/Gastrointestinal Agents; 0/Imidazoles; 0/Nitriles; 135017-85-5/KW 5092; 51-84-3/Acetylcholine

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