Document Detail


Oral delivery of double-stranded RNAs and siRNAs induces RNAi effects in the potato/tomato psyllid, Bactericerca cockerelli.
MedLine Citation:
PMID:  22110747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The potato/tomato psyllid, Bactericerca cockerelli (B. cockerelli), and the Asian citrus psyllid, Diaphorina citri (D. citri), are very important plant pests, but they are also vectors of phloem-limited bacteria that are associated with two devastating plant diseases. B. cockerelli is the vector of Candidatus Liberibacter psyllaurous (solanacearum), which is associated with zebra chip disease of potatoes, and D. citri is the vector of Ca. Liberibacter asiaticus, which is associated with the Huanglongbing (citrus greening) disease that currently threatens the entire Florida citrus industry. Here we used EST sequence information from D. citri to identify potential targets for RNA interference in B. cockerelli. We targeted ubiquitously expressed and gut-abundant mRNAs via injection and oral acquisition of double-stranded RNAs and siRNAs and were able to induce mortality in recipient psyllids. We also showed knockdown of target mRNAs, and that oral acquisition resulted primarily in mRNA knockdown in the psyllid gut. Concurrent with gene knockdown was the accumulation of target specific ∼ 21 nucleotide siRNAs for an abundant mRNA for BC-Actin. These results showed that RNAi can be a powerful tool for gene function studies in psyllids, and give support for continued efforts for investigating RNAi approaches as possible tools for psyllid and plant disease control.
Authors:
Hada Wuriyanghan; Cristina Rosa; Bryce W Falk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-16
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-11-23     Completed Date:  2012-03-26     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e27736     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, University of California Davis, Davis, California, United States of America.
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animal Feed
Animals
Base Sequence
Down-Regulation / genetics
Hemiptera / genetics*
Insect Proteins / deficiency,  genetics
Lycopersicon esculentum
Microinjections
Organ Specificity
Plants*
RNA Interference*
RNA, Double-Stranded / administration & dosage*,  genetics*
RNA, Messenger / genetics
RNA, Small Interfering / administration & dosage*,  genetics*
Solanum tuberosum
Transcriptome
Chemical
Reg. No./Substance:
0/Insect Proteins; 0/RNA, Double-Stranded; 0/RNA, Messenger; 0/RNA, Small Interfering
Comments/Corrections

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