Document Detail


Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats.
MedLine Citation:
PMID:  23283335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Relapse to maladaptive eating habits during dieting is often provoked by stress. Recently, we identified a role of dorsal medial prefrontal cortex (mPFC) neurons in stress-induced reinstatement of palatable food seeking in male rats. It is unknown whether endogenous neural activity in dorsal mPFC drives stress-induced reinstatement in female rats. Here, we used an optogenetic approach, in which female rats received bilateral dorsal mPFC microinjections of viral constructs coding light-sensitive eNpHR3.0-eYFP or control eYFP protein and intracranial fiber optic implants. Rats were food restricted and trained to lever press for palatable food pellets. Subsequently, pellets were removed, and lever pressing was extinguished; then the effect of bilateral dorsal mPFC light delivery on reinstatement of food seeking was assessed after injections of the pharmacological stressor yohimbine (an α-2 andrenoceptor antagonist) or pellet priming, a manipulation known to provoke food seeking in hungry rats. Dorsal mPFC light delivery attenuated yohimbine-induced reinstatement of food seeking in eNpHR3.0-injected but not eYFP-injected rats. This optical manipulation had no effect on pellet-priming-induced reinstatement or ongoing food-reinforced responding. Dorsal mPFC light delivery attenuated yohimbine-induced Fos immunoreactivity and disrupted neural activity during in vivo electrophysiological recording in awake rats. Optical stimulation caused significant outward currents and blocked electrically evoked action potentials in eNpHR3.0-injected but not eYFP-injected mPFC hemispheres. Light delivery alone caused no significant inflammatory response in mPFC. These findings indicate that intracranial light delivery in eNpHR3.0 rats disrupts endogenous dorsal mPFC neural activity that plays a role in stress-induced relapse to food seeking in female rats.
Authors:
Donna J Calu; Alex B Kawa; Nathan J Marchant; Brittany M Navarre; Mark J Henderson; Billy Chen; Hau-Jie Yau; Jennifer M Bossert; Geoffrey Schoenbaum; Karl Deisseroth; Brandon K Harvey; Bruce T Hope; Yavin Shaham
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  33     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-03     Completed Date:  2013-03-12     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  214-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Eating / drug effects,  physiology*
Extinction, Psychological / drug effects*
Feeding Behavior / drug effects,  physiology*
Female
Inhibition (Psychology)
Optogenetics
Prefrontal Cortex / drug effects,  physiology*
Rats
Rats, Long-Evans
Reinforcement (Psychology)
Self Administration
Stress, Psychological / physiopathology*
Yohimbine / pharmacology
Grant Support
ID/Acronym/Agency:
T32 DA007281/DA/NIDA NIH HHS; Z99 DA999999/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
2Y49VWD90Q/Yohimbine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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