Document Detail

Optogenetic control of cardiac function.
MedLine Citation:
PMID:  21071670     Owner:  NLM     Status:  MEDLINE    
The cardiac pacemaker controls the rhythmicity of heart contractions and can be substituted by a battery-operated device as a last resort. We created a genetically encoded, optically controlled pacemaker by expressing halorhodopsin and channelrhodopsin in zebrafish cardiomyocytes. Using patterned illumination in a selective plane illumination microscope, we located the pacemaker and simulated tachycardia, bradycardia, atrioventricular blocks, and cardiac arrest. The pacemaker converges to the sinoatrial region during development and comprises fewer than a dozen cells by the time the heart loops. Perturbation of the activity of these cells was entirely reversible, demonstrating the resilience of the endogenous pacemaker. Our studies combine optogenetics and light-sheet microscopy to reveal the emergence of organ function during development.
Aristides B Arrenberg; Didier Y R Stainier; Herwig Baier; Jan Huisken
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  330     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-12     Completed Date:  2010-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  971-4     Citation Subset:  IM    
Department of Physiology, University of California, San Francisco, CA 94158, USA.
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MeSH Terms
Animals, Genetically Modified
Atrioventricular Block / physiopathology
Bradycardia / physiopathology
Embryo, Nonmammalian / physiology
Embryonic Development
Halorhodopsins / genetics,  metabolism
Heart / embryology,  growth & development,  physiology*
Heart Arrest / physiopathology
Heart Conduction System / cytology,  embryology,  physiology*
Heart Rate*
Myocardial Contraction
Myocytes, Cardiac / metabolism
Rhodopsin / genetics,  metabolism
Sinoatrial Node / cytology,  physiology*
Tachycardia / physiopathology
Zebrafish / embryology,  genetics,  growth & development,  physiology*
Grant Support
Reg. No./Substance:
0/Halorhodopsins; 9009-81-8/Rhodopsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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