| Optogenetic analysis of synaptic function. | |
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MedLine Citation:
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PMID: 18794862 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We introduce optogenetic investigation of neurotransmission (OptIoN) for time-resolved and quantitative assessment of synaptic function via behavioral and electrophysiological analyses. We photo-triggered release of acetylcholine or gamma-aminobutyric acid at Caenorhabditis elegans neuromuscular junctions using targeted expression of Chlamydomonas reinhardtii Channelrhodopsin-2. In intact Channelrhodopsin-2 transgenic worms, photostimulation instantly induced body elongation (for gamma-aminobutyric acid) or contraction (for acetylcholine), which we analyzed acutely, or during sustained activation with automated image analysis, to assess synaptic efficacy. In dissected worms, photostimulation evoked neurotransmitter-specific postsynaptic currents that could be triggered repeatedly and at various frequencies. Light-evoked behaviors and postsynaptic currents were significantly (P <or= 0.05) altered in mutants with pre- or postsynaptic defects, although the behavioral phenotypes did not unambiguously report on synaptic function in all cases tested. OptIoN facilitates the analysis of neurotransmission with high temporal precision, in a neurotransmitter-selective manner, possibly allowing future investigation of synaptic plasticity in C. elegans. |
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Authors:
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Jana F Liewald; Martin Brauner; Greg J Stephens; Magali Bouhours; Christian Schultheis; Mei Zhen; Alexander Gottschalk |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-09-14 |
Journal Detail:
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Title: Nature methods Volume: 5 ISSN: 1548-7105 ISO Abbreviation: Nat. Methods Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-30 Completed Date: 2008-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101215604 Medline TA: Nat Methods Country: United States |
Other Details:
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Languages: eng Pagination: 895-902 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, Department of Biochemistry, Chemistry and Pharmacy, Goethe University Frankfurt, Biocenter N220, Frankfurt, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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physiology Animals Animals, Genetically Modified Caenorhabditis elegans / physiology Carrier Proteins / genetics, physiology Light* Motor Neurons / physiology Muscle Contraction Muscle Relaxation Synapses / physiology* Synaptic Transmission gamma-Aminobutyric Acid / physiology |
| Grant Support | |
ID/Acronym/Agency:
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P50 MH062196/MH/NIMH NIH HHS; R01 EY017241/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/channelopsin-2, Chlamydomonas reinhardtii; 51-84-3/Acetylcholine; 56-12-2/gamma-Aminobutyric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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