Document Detail


Optofluidic device for label-free cell classification from whole blood.
MedLine Citation:
PMID:  22875178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrated a unique optofluidic lab-on-a-chip device that can measure optically encoded forward scattering signals. From the design of the spatial pattern, we can measure the position and velocity of each cell in the flow and generate a 2-D cell distribution plot over the cross section of the channel. Moreover, we have demonstrated that the cell distribution is highly sensitive to its size and stiffness. The latter is an important biomarker for cell classification and our method offers a simple and unequivocal method to classify cells by their size and stiffness. We have proved the concept using live and fixed HeLa cells. Due to the stiffness and size difference of neutrophils compared to other types of white blood cells, we have demonstrated detection of neutrophils from other blood cells. Finally, we have performed the test using 5 μL of human blood. In a greatly simplified blood preparation process, skipping the usual steps of anticoagulation, centrifuge, antibody labelling or staining, filtering, etc., we have demonstrated that our device and detection principle can count neutrophils in whole human blood. Our system is compact, inexpensive and simple to fabricate and operate, having a commodity laser diode and a Si PIN photoreceiver as the main pieces of hardware. Although the results are still preliminary, the studies indicate that this optofluidic device holds promise to be a point-of-care and home care device to measure neutrophil concentration, which is the key indicator of the immune functions for cancer patients undergoing chemotherapy.
Authors:
Tsung-Feng Wu; Zhe Mei; Yu-Hwa Lo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-08-23     Completed Date:  2012-12-28     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  3791-7     Citation Subset:  IM    
Affiliation:
Materials Science Program, University of California at San Diego, La Jolla, California 92093-0418, USA. tsungfong@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Blood Cells / cytology
Cell Size
HeLa Cells
Humans
Lab-On-A-Chip Devices
Lasers
Microfluidic Analytical Techniques / instrumentation*,  methods
Neutrophils / cytology*
Optics and Photonics / instrumentation*
Silicon / chemistry
Grant Support
ID/Acronym/Agency:
1R21RR024453/RR/NCRR NIH HHS; 1R43RR032225/RR/NCRR NIH HHS; R21 RR024453/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
7440-21-3/Silicon
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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