Document Detail

Optimum extraction process of polyphenols from Bridelia grandis stem bark using experimental design.
MedLine Citation:
PMID:  20309897     Owner:  NLM     Status:  MEDLINE    
Euphorbiaceae barks are known to contain an appreciable amount of polyphenolic compounds responsible for several biological activities. Preliminary extraction from Bridelia grandis stem bark afforded high content of polyphenols, determined by spectrophotometric methods such as Folin-Ciocalteu (for total phenols, TP) and n-butanol-HCl (for condensed tannins, CT). A preliminary Plackett-Burman screening design was used to identify the key factors that influence the TP and CT extraction. Between all the variables known to influence the extraction from vegetable matrixes, six were selected; maceration was chosen as traditional extraction methodology. To investigate the effect of solvents and extraction method, methanol, acetone 70% (v/v in water), centrifugation and ultrasound were chosen. A full factorial design 2(3) was applied to optimize the extraction procedure. The responses were obtained analyzing the extracts for their TP and CT contents determined by the above-mentioned spectrophotometric methods. The results confirm that, within the explored domain, the optimum solvent is methanol and the optimum method is one-cycle centrifugation. Finally, it was also compared with the effect of maceration on the considered responses. It has never given results better than centrifugation, whereas in the case of CT it represents an advantage to employ a three-cycle centrifugation instead of one.
Gloria Brusotti; Tatiana Adrienne Ngueyem; Raffaela Biesuz; Gabriele Caccialanza
Related Documents :
20188977 - Accelerated solvent extraction for gc-based tobacco fingerprinting and its comparison w...
21703677 - Comparative study of digestion methods epa 3050b (hno(3)-h(2)o(2)-hcl) and iso 11466.3 ...
25461697 - Metabolite profiling elucidates communalities and differences in the polyphenol biosynt...
16245067 - Is dimethylsulfoxide a reliable solvent for extracting chlorophyll under field conditions?
15273977 - Quantitative analysis of phytoestrogens in kudzu-root, soy and spiked serum samples by ...
8720907 - Coulometric detection in high-performance liquid chromatographic analysis of cholestery...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of separation science     Volume:  33     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1692-7     Citation Subset:  IM    
Department of Pharmaceutical Chemistry, University of Pavia, Pavia, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Butanols / chemistry
Chemistry Techniques, Analytical
Flavonoids / analysis
Medicine, Traditional
Phenols / analysis
Phyllanthus / metabolism*
Plant Bark / metabolism*
Plant Extracts / analysis,  isolation & purification*
Reg. No./Substance:
0/Butanols; 0/Flavonoids; 0/Phenols; 0/Plant Extracts; 0/Solvents; 0/polyphenols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Use of different buffers for detection and separation in determination of physio-active components i...
Next Document:  Ionic liquid as extraction agent for detection of volatile phenols in wastewater and its regeneratio...