| Optimum extraction process of polyphenols from Bridelia grandis stem bark using experimental design. | |
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MedLine Citation:
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PMID: 20309897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Euphorbiaceae barks are known to contain an appreciable amount of polyphenolic compounds responsible for several biological activities. Preliminary extraction from Bridelia grandis stem bark afforded high content of polyphenols, determined by spectrophotometric methods such as Folin-Ciocalteu (for total phenols, TP) and n-butanol-HCl (for condensed tannins, CT). A preliminary Plackett-Burman screening design was used to identify the key factors that influence the TP and CT extraction. Between all the variables known to influence the extraction from vegetable matrixes, six were selected; maceration was chosen as traditional extraction methodology. To investigate the effect of solvents and extraction method, methanol, acetone 70% (v/v in water), centrifugation and ultrasound were chosen. A full factorial design 2(3) was applied to optimize the extraction procedure. The responses were obtained analyzing the extracts for their TP and CT contents determined by the above-mentioned spectrophotometric methods. The results confirm that, within the explored domain, the optimum solvent is methanol and the optimum method is one-cycle centrifugation. Finally, it was also compared with the effect of maceration on the considered responses. It has never given results better than centrifugation, whereas in the case of CT it represents an advantage to employ a three-cycle centrifugation instead of one. |
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Authors:
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Gloria Brusotti; Tatiana Adrienne Ngueyem; Raffaela Biesuz; Gabriele Caccialanza |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of separation science Volume: 33 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-31 Completed Date: 2010-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 1692-7 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Chemistry, University of Pavia, Pavia, Italy. gloria.brusotti@unipv.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Butanols
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chemistry Centrifugation Chemistry Techniques, Analytical Flavonoids / analysis Medicine, Traditional Phenols / analysis Phyllanthus / metabolism* Plant Bark / metabolism* Plant Extracts / analysis, isolation & purification* Solvents Spectrophotometry Temperature Ultrasonics |
| Chemical | |
Reg. No./Substance:
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0/Butanols; 0/Flavonoids; 0/Phenols; 0/Plant Extracts; 0/Solvents; 0/polyphenols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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