Document Detail


Optimizing vector application for gene transfer into human hepatoblastoma cells.
MedLine Citation:
PMID:  16896819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gene targeting is currently of distinct interest as an innovative additive treatment option in various malignancies. Its role in pediatric liver tumors has not yet been evaluated thoroughly. For the first time the authors systematically analyzed both lipid-based transfection as well as transduction with adenovirus vectors (Ad) and Sendai virus vectors (SeVV) in order to optimize gene transfer into hepatoblastoma (HB) cells. Two HB cell lines were infected with Ad or SeVV coding for green fluorescent protein (Ad-GFP, SeVV-GFP); transduction efficiencies and apoptosis were assessed using flow cytometry. Furthermore, lipofection of HB cell lines with plasmid-constructs comprising liver-specific promoters was performed using Lipofectamine 2000 and FuGENE 6; lipofection efficiency was monitored by flow cytometry, microscopy, and luciferase activity. The Ad-GFP showed higher transduction rates (61-86%) than the SeVV-GFP (4-24%) depending on the HB cell line used. Infections with first generation SeVV vectors (SeVV-GFP) led to increased target cell apoptosis (7-43%) compared to Ad-GFP (4-16%). The Lipofectamine 2000 revealed a higher transfection efficiency than the FuGENE 6 for both HB cell lines tested. The liver-specific promoters were found to be differently active in the HB cell lines. This study delineates recombinant adenovirus vectors as a promising tool for gene transduction in the HB cells. Furthermore, enhanced activity of the liver-specific promoters in HUH6 cells compared to HepT1 cells supports the observation of varying biological behavior in histologically differing HB tissues.
Authors:
Steven W Warmann; Sorin Armeanu; Heike Heitmann; Peter Ruck; Guido Seitz; Johannes T Wessels; Marie-Luise Lemken; Ulrich M Lauer; Jörg Fuchs; Michael Bitzer
Related Documents :
15629439 - Characterization of the mutant (a115v) tissue-nonspecific alkaline phosphatase gene fro...
20416459 - In vitro expression and characterization of myh9 mutant alleles linked to hereditary he...
18374229 - Combination of pten and gamma-ionizing radiation enhances cell death and g(2)/m arrest ...
19815039 - How to study dendriplexes ii: transfection and cytotoxicity.
25181289 - Uncoupling different characteristics of the c. elegans e lineage from differentiation o...
16237759 - Effect of snc19/st14 gene overexpression on invasion of colorectal cancer cells.
24734929 - Nano-hydroxyapatite and nano-titanium dioxide exhibit different sub-cellular distributi...
12197769 - Spontaneous differentiation of porcine and bovine embryonic stem cells (epiblast) into ...
22841689 - In vivo biomarker expression patterns are preserved in 3d cultures of prostate cancer.
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article     Date:  2006-07-29
Journal Detail:
Title:  Pediatric surgery international     Volume:  22     ISSN:  0179-0358     ISO Abbreviation:  Pediatr. Surg. Int.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-31     Completed Date:  2007-01-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8609169     Medline TA:  Pediatr Surg Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  733-42     Citation Subset:  IM    
Affiliation:
Department of Pediatric Surgery, University of Tübingen, Hoppe-Seyler-Str.3, 72076, Tübingen, Germany. Steven.Warmann@med.uni-tuebingen.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenoviridae
Cell Line, Tumor
Drug Carriers*
Genetic Vectors*
Green Fluorescent Proteins
Hepatoblastoma / therapy*
Humans
Lipids
Liver Neoplasms / therapy*
Promoter Regions, Genetic
Sendai virus
Transfection / methods*
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/FuGene; 0/Lipids; 0/Lipofectamine; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A hepaticojejunostomy: technical errors with 'twists and turns'.
Next Document:  Lytic rib lesion in a 1-year-old child: group A beta streptococcal osteomyelitis mimicking tumor.