Document Detail


Optimizing the use of sex-sorted sperm in timed artificial insemination programs for suckled beef cows1.
MedLine Citation:
PMID:  22205672     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Three experiments were designed to evaluate methods to optimize the use of sex-sorted sperm in timed AI (TAI) programs for suckled beef cows. In all 3 experiments, suckled Bos indicus cows were synchronized using an intravaginal progesterone (P4) device during 8 d and a 2.0-mg injection of i.m. estradiol benzoate (EB) at device insertion. The females received PG and equine chorionic gonadotropin (300 IU) at P4 device removal and 1.0 mg of EB 24 h later. The cows were timed inseminated 60 to 64 h after P4 device withdrawal. All cows had their ovaries scanned by transrectal ultrasound at TAI to indentify and to measure the largest follicle (LF) present. In Experiment 1, a total of 853 cows had their LF classified as < 9 mm or ≥ 9 mm at the time of TAI; these cows were then randomly assigned to 4 groups according to their LF diameter (< 9 mm or ≥ 9 mm) and the type of sperm used (sex-sorted or non-sex-sorted). There was an interaction (P = 0.02) between the type of sperm and LF diameter beginning at TAI (non-sex-sorted ≥ 9 mm = 58.9%(a) [126/214]; non-sex-sorted < 9 mm = 49.5%(b) [106/214]; sex-sorted ≥ 9 mm = 56.8%(ab) [134/236]; and sex-sorted < 9 mm = 31.2%(c) [59/189]). In Experiment 2, suckled cows (n = 491) were classified immediately before TAI as having displayed estrus or not (estrus or no estrus),, between P4 device removal and TAI. These cows were randomly assigned to 4 groups according to the occurrence of estrus and the type of sperm (sex-sorted or non-sex-sorted). There were effects of the occurrence of estrus (P = 0.0003) and the type of sperm (P = 0.05) on pregnancy per AI (P/AI; no estrus, non-sex-sorted = 43.6% [27/62]; estrus, non-sex-sorted = 58.5%; [107/183]; no estrus, sex-sorted = 33.9% [21/62], and estrus, sex-sorted = 50.0% [92/184]); however, no interaction between the occurrence of estrus and type of sperm was observed (P = 0.87). In Experiment 3, a total of 200 suckled cows presenting LF ≥ 9 mm at TAI were randomly assigned to receive sex-sorted sperm deposited into the uterine body (n = 100) or into the uterine horn ipsilateral to the recorded LF (n = 100). No effect of deeper artificial insemination on P/AI was found (P = 0.57). Therefore, the LF diameter at TAI and the occurrence of estrus can be used as selection criteria to identify cows with higher odds of pregnancy to receive sex-sorted sperm in TAI programs. In addition, performing TAI with sex-sorted sperm deeper into the uterus did not alter the pregnancy results.
Authors:
M F Sá Filho; R Girotto; E K Abe; L Penteado; E P Campos Filho; J F Moreno; R V Sala; M Nichi; P S Baruselli
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-28
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Animal Reproduction, FMVZ-USP, Prof. Orlando Marques de Paiva, 87, 05508-270, Sao Paulo, SP, Brazil.
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