Document Detail

Optimizing signal and mass resolution for matrix-assisted laser desorption utilizing a linear time-of-flight mass spectrometer.
MedLine Citation:
PMID:  1334736     Owner:  NLM     Status:  MEDLINE    
Results are presented for various instrumental configurations employed for matrix-assisted laser desorption mass spectrometry. Mass resolution is determined for a linear time-of-flight mass spectrometer for various lengths of the field-free region. A wire ion guide is utilized and is shown to improve ion transport efficiencies for longer field-free regions. It is also determined experimentally that a modest mass resolution increase is often obtained in configurations employing the wire ion guide when compared to the mass resolution obtained with the same geometry without the wire ion guide. Optimal applied potentials are determined for the wire ion guide. No mass dependence on the optimal applied potential (-100 V) for the wire ion guide is observed for samples of equine myoglobin (MW 16,951.5 Da) and a bacterial protease (MW 27,228.4 Da). The optimal applied voltage was also found to be identical (-100 V) for the singly through quadruply charged molecular ion species of rabbit gamma globulin (MW approximately 150,000 Da). It is shown that a 2 m flight tube with a wire ion guide provides better signal-to-noise mass spectra than a 1 m flight tube without the wire ion guide and can more than double the mass resolution obtainable. Utilization of a 4 m flight tube gives minimal mass resolution enhancement at the expense of signal-to-noise.
R S Brown; N L Gilfrich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  6     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-01-22     Completed Date:  1993-01-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  697-701     Citation Subset:  IM    
Department of Chemistry, Colorado State University, Fort Collins 80523.
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MeSH Terms
Coumaric Acids
Cytochrome c Group / analysis
Indicators and Reagents
Insulin / analysis
Mass Spectrometry / instrumentation,  methods*
Myoglobin / analysis
Serum Albumin, Bovine / analysis
Subtilisins / analysis
Grant Support
Reg. No./Substance:
0/Coumaric Acids; 0/Cytochrome c Group; 0/Indicators and Reagents; 0/Myoglobin; 0/Serum Albumin, Bovine; 11061-68-0/Insulin; 28166-41-8/alpha-cyano-4-hydroxycinnamate; 530-59-6/sinapinic acid; EC 3.4.21.-/Subtilisins

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