Document Detail


Optimizing separation conditions for polycyclic aromatic hydrocarbons in micellar electrokinetic chromatography.
MedLine Citation:
PMID:  11521889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the separation of polycyclic aromatic hydrocarbons (PAHs) using 0.1% poly(ethylene oxide) (PEO) in micellar electrokinetic chromatography (MEKC). In the presence of PEO, adsorption of PAHs on the capillary wall was reduced, leading to better resolution and reproducibility. Effects of tetrapentylammonium iodide (TPAI), dextran sulfate (DS), methanol, and sodium lauryl sulfate (SDS) on the separation of PAHs were elucidated. In terms of resolution and speed, DS, compared to TPAI, is a better additive for separation of PAHs. When using 0.1% PEO solution containing 45% methanol, 50 mM SDS, and 0.02% DS, separation of 10 PAHs containing 2 to 5 benzene rings was accomplished in less than 12 min at 15 kV in a commercial CE system. The method has also been tested for separating seven PAHs with high quantum yields when excited at 325 nm using a He-Cd laser. Unfortunately, separation of the seven PAHs was not achieved and sensitivity diminished under the same conditions. To optimize sensitivity, resolution and speed, a stepwise technique in MEKC has been proposed. The seven PAHs were resolved in 35 min at 15 kV when separation was performed in 0.1% PEO solution containing 35 mM SDS, 40% methanol and 0.02% DS for 2 min, and subsequently in 0.1% PEO solution containing 20 mM SDS, 50% methanol, and 0.02% DS.
Authors:
M M Hsieh; Y C Kuo; P L Tsai; H T Chang
Related Documents :
10985559 - Solid-phase extraction of sugar cane soot extract for analysis by gas chromatography wi...
11605749 - Double molecular imprinting--a new sensor concept for improving selectivity in the dete...
3131379 - High-performance liquid chromatographic method for isolating organic contaminants from ...
16235789 - Study of thermal behavior of vitamin d3 by pyrolysis-gc-ms in combination with boiling ...
18816459 - High-performance liquid chromatography determination of nitrated polycyclic aromatic hy...
23943279 - Identification of (z)-4- and 1-tridecene in the metathoracic gland secretions of stink ...
20334179 - Determination of aflatoxins in botanical roots by a modification of aoac official metho...
24226299 - Isolation and partial characterization of a novel keratan sulfate proteoglycan from met...
22052789 - Determination of ethylene glycol dinitrate in dynamites using hplc: application to the ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  924     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-08-27     Completed Date:  2002-01-29     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  397-405     Citation Subset:  IM    
Affiliation:
Department of Chemistry, National Taiwan University, Taipei.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, Micellar Electrokinetic Capillary / methods*
Polycyclic Compounds / isolation & purification*
Sensitivity and Specificity
Sodium Dodecyl Sulfate
Chemical
Reg. No./Substance:
0/Polycyclic Compounds; 151-21-3/Sodium Dodecyl Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Analysis of post-harvest fungicides by micellar electrokinetic chromatography.
Next Document:  Liquid-liquid-liquid microextraction of nitrophenols with a hollow fiber membrane prior to capillary...