Document Detail

Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases.
MedLine Citation:
PMID:  19880314     Owner:  NLM     Status:  MEDLINE    
A low level of phosphoric acid (1% w/w on dry bagasse basis, 160 degrees C and above, 10 min) was shown to effectively hydrolyze the hemicellulose in sugar cane bagasse into monomers with minimal side reactions and to serve as an effective pre-treatment for the enzymatic hydrolysis of cellulose. Up to 45% of the remaining water-insoluble solids (WIS) was digested to sugar monomers by a low concentration of Biocellulase W (0.5 filter paper unit/gWIS) supplemented with beta-glucosidase, although much higher levels of cellulase (100-fold) were required for complete hydrolysis. After neutralization and nutrient addition, phosphoric acid syrups of hemicellulose sugars were fermented by ethanologenic Escherichia coli LY160 without further purification. Fermentation of these syrups was preceded by a lag that increased with increased pre-treatment temperature. Further improvements in organisms and optimization of steam treatments may allow the co-fermentation of sugars derived from hemicellulose and cellulose, eliminating need for liquid-solid separation, sugar purification, and separate fermentations.
C C Geddes; J J Peterson; C Roslander; G Zacchi; M T Mullinnix; K T Shanmugam; L O Ingram
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-10-31
Journal Detail:
Title:  Bioresource technology     Volume:  101     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1851-7     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Elsevier Ltd. All rights reserved.
Department of Microbiology and Cell Science, University of Florida, Box 110700, Gainesville, FL 32611, United States.
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MeSH Terms
Biotechnology / methods*
Carbohydrates / chemistry
Cellulase / chemistry*
Cellulose / chemistry*
Ethanol / chemistry
Fungi / enzymology*
Lignin / chemistry
Phosphoric Acids / chemistry*
Saccharum / chemistry
Sulfuric Acids / chemistry
Time Factors
Xylose / chemistry
Reg. No./Substance:
0/Carbohydrates; 0/Phosphoric Acids; 0/Sulfuric Acids; 0/Xylose; 11132-73-3/lignocellulose; 64-17-5/Ethanol; 7664-38-2/phosphoric acid; 7664-93-9/sulfuric acid; 9004-34-6/Cellulose; 9005-53-2/Lignin; 9006-97-7/bagasse; EC

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