Document Detail


Optimizing probucol administration to preserve left ventricular compliance after reperfusion injury in the heterotopic rat heart isograft.
MedLine Citation:
PMID:  12957605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We investigated the optimal method of administering probucol to attenuate reperfusion-induced diastolic abnormalities in the left ventricle of the heterotopically transplanted rat heart isograft. METHODS: We assigned Lewis rats (n = 84) to 7 transplant groups. We arrested 42 hearts using coronary perfusion with hypothermic University of Wisconsin (UW) solution at 60 mm Hg and abdominally isografted the hearts. Neither donors nor recipients in the control group (C-C, n = 6) received probucol. Oral probucol (1% by weight in chow) was fed for 1 month before surgery to the donors only (OP-C, n = 6), the recipients only (C-OP, n = 6), or both (OP-OP, n = 6). We administered an intraperitoneal injection of probucol (300 mg/kg) in 3 ml oil 1 hour before surgery to the donors only (IP-C, n = 6), the recipients only (C-IP, n = 6), or both (IP-IP, n = 6). Transplanted hearts were reperfused for 15 minutes and re-arrested. We also arrested the control recipients' native hearts (native, n = 6). We measured post-mortem left ventricular compliance curves and myocardial water content. RESULTS: The most compliant grafts were in Groups IP-IP, OP-OP, and C-IP. All isograft groups had significantly less left ventricular compliance than did native hearts. Myocardial water content was significantly greater in controls than in natives, OP-OP, and OP-C. CONCLUSIONS: Pre-treatment with intraperitoneal probucol in cardiac isograft recipients combines optimal protection with simple administration when UW solution is used for both arrest and preservation. Further studies of the protective effect of probucol against reperfusion injury in large-animal models are warranted and should use intraperitoneal or intravenous injection in recipients.
Authors:
David G Rabkin; Alan D Weinberg; Henry M Spotnitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation     Volume:  22     ISSN:  1053-2498     ISO Abbreviation:  J. Heart Lung Transplant.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-05     Completed Date:  2005-04-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9102703     Medline TA:  J Heart Lung Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  959-66     Citation Subset:  IM    
Affiliation:
Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animals
Antioxidants / administration & dosage*,  therapeutic use
Disease Models, Animal
Heart Transplantation*
Myocardial Reperfusion Injury / prevention & control*
Premedication
Probucol / administration & dosage*,  therapeutic use
Rats
Rats, Inbred Lew
Transplantation, Isogeneic
Ventricular Function, Left / drug effects*
Grant Support
ID/Acronym/Agency:
F32 HL69641-01/HL/NHLBI NIH HHS; R01 HL48109/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 23288-49-5/Probucol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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