| Optimizing photodynamic therapy by liposomal formulation of the photosensitizer pyropheophorbide-a methyl ester: in vitro and ex vivo comparative biophysical investigations in a colon carcinoma cell line. | |
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MedLine Citation:
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PMID: 20714673 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photodynamic therapy (PDT), induced by a photosensitizer (PS) encapsulated in a nanostructure, has emerged as an appropriate treatment to cure a multitude of oncological and non-oncological diseases. Pyropheophorbide-a methyl ester (PPME) is a second-generation PS tested in PDT, and is a potential candidate for future clinical applications. The present study, carried out in a human colon carcinoma cell line (HCT-116), evaluates the improvement resulting from a liposomal formulation of PPME versus free-PPME. Absorption and fluorescence spectroscopies, fluorescence lifetime measurements, subcellular imaging and co-localization analysis have been performed in order to analyze the properties of PPME for each delivery mode. The benefit of drug encapsulation in DMPC-liposomes is clear from our experiments, with a 5-fold higher intracellular drug delivery than that observed with free-PPME at similar concentrations. The reactive oxygen species (ROSs) produced after PPME-mediated photosensitization have been identified and quantified by using electron spin resonance spectroscopy. Our results demonstrate that PPME-PDT-mediated ROSs are composed of singlet oxygen and a hydroxyl radical. The small amounts of PPME inside mitochondria, as revealed by fluorescence co-localization analysis, could maybe explain the very low apoptotic cell death measured in HCT-116 cells. |
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Authors:
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Pierre-Henri Guelluy; Marie-Pierre Fontaine-Aupart; Angeliki Grammenos; Sandrine Lécart; Jacques Piette; Maryse Hoebeke |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2010-08-12 |
Journal Detail:
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Title: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology Volume: 9 ISSN: 1474-9092 ISO Abbreviation: Photochem. Photobiol. Sci. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-25 Completed Date: 2010-12-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101124451 Medline TA: Photochem Photobiol Sci Country: England |
Other Details:
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Languages: eng Pagination: 1252-60 Citation Subset: IM |
Affiliation:
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Laboratory of Biomedical Spectroscopy, Institute of Physics, B5a, University of Liège, Sart-Tilman, B-4000, Liège, Belgium. phguelluy@ulg.ac.be |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma
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drug therapy Cell Line, Tumor Colonic Neoplasms / drug therapy Dimyristoylphosphatidylcholine / chemistry Electron Spin Resonance Spectroscopy Humans Liposomes / chemistry Microscopy, Confocal Mitochondria / drug effects Photochemotherapy Photosensitizing Agents / administration & dosage*, chemistry, therapeutic use Porphyrins / administration & dosage*, chemistry, therapeutic use Reactive Oxygen Species / metabolism Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; 0/Photosensitizing Agents; 0/Porphyrins; 0/Reactive Oxygen Species; 0/pyropheophorbide-a methylester; 13699-48-4/Dimyristoylphosphatidylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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