Document Detail

Optimizing orthotopic cell transplantation in the mouse adrenal gland.
MedLine Citation:
PMID:  20525431     Owner:  NLM     Status:  MEDLINE    
Orthotopic cell transplantation models are important for a complete understanding of cell-cell interactions as well as tumor biology. In published studies of orthotopic transplantation in the mouse adrenal gland, human neuroblastoma cells have been shown to invade and occupy the adrenal, but in these investigations a true orthotopic model was not established. Here we show an orthotopic model in which transplanted cells are retained within the adrenal gland by formation of a fibrin clot. To establish an appropriate technique, we used brightly fluorescent 10 microm polystyrene microspheres injected into the mouse adrenal gland. In the absence of fibrinogen/thrombin for clot formation, much of the injected material was extruded to the outside of the gland. When the microspheres were injected in a fibrinogen/thrombin mixture, fluorescence was confined to the adrenal gland. As a model neoplastic cell originating from the cortex of the gland, we used a tumorigenic bovine adrenocortical cell line. When 3 x 10(5) cells were implanted orthotopically, by 16 days the cell mass had expanded and had invaded the cortex, whereas when 1 x 10(5) cells were used, tumor masses were much smaller. We therefore subsequently used 3 x 10(5) cells. When mice were sacrificed at different time points, we found that tumor growth resulting was progressive and that by 26 days cells there was extensive invasion into the cortex or almost complete replacement of the cortex with tumor cells. As a model neoplastic cell of neural crest origin, we used SK-N-AS human neuroblastoma cells. Orthotopic transplantation of 3 x 10(5) cells resulted in extensive invasion and destruction of the gland by 26 days. In summary, the present orthotopic model for intra-adrenal cell transplantation is valuable for investigation of growth of neoplastic cells of both cortical and medullary origin and should be useful for future studies of cortex-medulla interactions.
Cibele C Cardoso; Stefan R Bornstein; Peter J Hornsby
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-03
Journal Detail:
Title:  Cell transplantation     Volume:  19     ISSN:  1555-3892     ISO Abbreviation:  Cell Transplant     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-11-22     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  565-72     Citation Subset:  IM    
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MeSH Terms
Adrenal Glands / cytology*,  metabolism
Cell Line, Tumor
Cell Transplantation / methods*
Cells, Cultured
Disease Models, Animal
Fibrin / metabolism
Fibrinogen / administration & dosage
Mice, Transgenic
Neoplasm Transplantation / methods*
Neuroblastoma / metabolism,  pathology*
Thrombin / administration & dosage
Transplantation, Heterologous / methods*
Grant Support
P01 AG020752/AG/NIA NIH HHS; P01 AG020752-020006/AG/NIA NIH HHS; R37 AG012287/AG/NIA NIH HHS; R37 AG012287-14/AG/NIA NIH HHS
Reg. No./Substance:
9001-31-4/Fibrin; 9001-32-5/Fibrinogen; EC

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