| Optimizing the medium perfusion rate in bone tissue engineering bioreactors. | |
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MedLine Citation:
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PMID: 21449028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is a critical need to increase the size of bone grafts that can be cultured in vitro for use in regenerative medicine. Perfusion bioreactors have been used to improve the nutrient and gas transfer capabilities and reduce the size limitations inherent to static culture, as well as to modulate cellular responses by hydrodynamic shear. Our aim was to understand the effects of medium flow velocity on cellular phenotype and the formation of bone-like tissues in three-dimensional engineered constructs. We utilized custom-designed perfusion bioreactors to culture bone constructs for 5 weeks using a wide range of superficial flow velocities (80, 400, 800, 1,200, and 1,800 µm/s), corresponding to estimated initial shear stresses ranging from 0.6 to 20 mPa. Increasing the flow velocity significantly affected cell morphology, cell-cell interactions, matrix production and composition, and the expression of osteogenic genes. Within the range studied, the flow velocities ranging from 400 to 800 µm/s yielded the best overall osteogenic responses. Using mathematical models, we determined that even at the lowest flow velocity (80 µm/s) the oxygen provided was sufficient to maintain viability of the cells within the construct. Yet it was clear that this flow velocity did not adequately support the development of bone-like tissue. The complexity of the cellular responses found at different flow velocities underscores the need to use a range of evaluation parameters to determine the quality of engineered bone. |
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Authors:
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Warren L Grayson; Darja Marolt; Sarindr Bhumiratana; Mirjam Fröhlich; X Edward Guo; Gordana Vunjak-Novakovic |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-12-22 |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 108 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-03-30 Completed Date: 2011-07-12 Revised Date: 2012-03-26 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 1159-70 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley Periodicals, Inc. |
Affiliation:
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Department of Biomedical Engineering, Columbia University, New York, New York 10032, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bioreactors* Bone and Bones / metabolism* Culture Media* Humans Perfusion Tissue Engineering* |
| Grant Support | |
ID/Acronym/Agency:
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DE161525/DE/NIDCR NIH HHS; EB02520/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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