Document Detail

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model.
MedLine Citation:
PMID:  22898801     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation
METHODS: Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured.
RESULTS: When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols.
CONCLUSION: Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.
Jian-Min Zhang; Ying-Chun Zhang; Li-Hong Ruan; Heng-Cai Wang
Publication Detail:
Type:  Journal Article     Date:  2012-08-17
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-06-06     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1255-60     Citation Subset:  IM    
Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, China, 250000.
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MeSH Terms
Cryopreservation / methods*
Cryoprotective Agents
Models, Animal
Organ Preservation / methods*
Ovarian Follicle / physiology,  ultrastructure
Ovary / cytology,  physiology*
Perfusion / instrumentation,  methods*
Reg. No./Substance:
0/Cryoprotective Agents

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