| Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model. | |
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MedLine Citation:
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PMID: 22898801 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation METHODS: Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured. RESULTS: When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols. CONCLUSION: Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation. |
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Authors:
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Jian-Min Zhang; Ying-Chun Zhang; Li-Hong Ruan; Heng-Cai Wang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-17 |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: - ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, China, 250000. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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