Document Detail

Optimizing Suture Middle Cerebral Artery Occlusion Model in C57BL/6 Mice Circumvents Posterior Communicating Artery Dysplasia.
MedLine Citation:
PMID:  22054164     Owner:  NLM     Status:  Publisher    
The suture middle cerebral artery occlusion model is used worldwide in both academia and industry. However, the variable occurrence of dysplasia in posterior communicating arteries (PcomAs) induces higher mortality and instability, limiting the model's application. In particular, high mortality in intraluminal suture MCAO models is associated with the dysplasia of PcomAs in C57BL/6 mice. Optimization of silicone coating length is critical for reducing mortality and generating stable infarct in this model. The aim of our study is to reduce mortality in and improve the reproducibility of the intraluminal suture middle cerebral artery occlusion (MCAO) model in C57BL/6 mice, which have high variation in PcomA dysplasia. Adult male C57BL/6 mice (n=38) underwent MCAO using sutures with various diameters and silicone coating lengths. The occlusion of cerebral vessels was examined by synchrotron radiation live angiography. The morphology of PcomAs was examined under a microscope after Microfil infusion. Neurological outcome, infarct volume, and mortality were studied within 28 days. Optimizing the silicone coating on an 8-0 suture tip, we were able to reduce the model mortality to zero in C57BL/6 and produce stable brain infarct volume independent of the patency of PcomAs.
Falei Yuan; Yaohui Tang; Xiaojie Lin; Yan Xi; Yongjing Guan; Tiqiao Xiao; Jun Chen; Zhijun Zhang; Guo-Yuan Yang; Yongting Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-5
Journal Detail:
Title:  Journal of neurotrauma     Volume:  -     ISSN:  1557-9042     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8811626     Medline TA:  J Neurotrauma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Neuroscience and Neuroengineering Center, Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China;
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