| Optimized multiparametric immunophenotyping of umbilical cord blood cells by flow cytometry. | |
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MedLine Citation:
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PMID: 20595961 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Umbilical cord blood is a key source of stem cells for transplantation and regenerative medicine. To maximize each cord blood sample, it is important to analyze its cellular populations. Many cord blood banks focus on counts of total nucleated cells and/or cells that carry the CD34 antigen, but this limited focus does not give a true estimation of cord blood content, quality and appropriateness for use. This protocol is the first of its kind to enable a comprehensive investigation of cord blood cellular populations. Using multicolor flow cytometry it is possible to examine expression of 26 antigens--including hematopoietic markers CD45 and CD34, immune markers CD19 and CD3, and HLA--using a total of only 1 x 10(6) cells from each unit for both pre- and post-processing. The samples are stained, lysed, washed and analyzed flow cytometrically. This method also provides valuable information beyond cord blood composition and quality; for example, it can also be used to assess whether maternal factors affect CD34(+) cell numbers. Collection, processing, cryopreservation and initial flow cytometry take approximately 5 h. |
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Authors:
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Christina Basford; Nico Forraz; Colin McGuckin |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-24 |
Journal Detail:
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Title: Nature protocols Volume: 5 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-02 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 1337-46 Citation Subset: IM |
Affiliation:
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Stem Cell Group, Institute of Human Genetics, Newcastle University, Newcastle upon Tyne, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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analysis* Blood Cells / immunology* Fetal Blood / immunology* Flow Cytometry / instrumentation, methods* Humans Immunophenotyping / methods* Infant, Newborn |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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