Document Detail


Optimized assay for serum angiotensin-converting enzyme activity.
MedLine Citation:
PMID:  6273013     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This optimized two-point kinetic assay for serum angiotensin-converting enzyme is based on the colorimetric determination of hippurate with cyanuric chloride/dioxan reagent. The hippurate is released from hippuryl-L-histidyl-L-leucine by angiotensin-converting enzyme in the presence of chloride ion. CVs for the method (within-run and between-run) ranged from 2.1 to 3.2%. Linearity extends up to 200 U/L. Results are unaffected by lipemia and icterus. Hemoglobin in concentrations greater than 1.5 g/L causes a slight negative interference. Reference intervals for men and women are 22-82 U/L and 25-69 U/L, respectively.
Authors:
P L Hurst; C J Lovell-Smith
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  27     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-02-25     Completed Date:  1982-02-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2048-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Buffers
Chemical Phenomena
Chemistry
Child
Child, Preschool
Colorimetry / methods
Female
Hippurates / metabolism
Humans
Hydrogen-Ion Concentration
Lung / enzymology
Male
Middle Aged
Oligopeptides / metabolism
Peptidyl-Dipeptidase A / blood*
Phosphates
Reference Values
Triazines
Chemical
Reg. No./Substance:
0/Buffers; 0/Hippurates; 0/Oligopeptides; 0/Phosphates; 0/Triazines; 108-77-0/cyanuric chloride; 31373-65-6/hippuryl-histidyl-leucine; EC 3.4.15.1/Peptidyl-Dipeptidase A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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