| Optimized Pathogen Detection with 30 Compared to 20 ml Blood Culture Draws. | |
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MedLine Citation:
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PMID: 21976759 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Using data from 23,313 patients, we assessed whether two blood culture sets of three bottles per set would detect more pathogens than two sets of two bottles per set, and achieve similar sensitivity to collecting three sets of two bottles per set. We also compared the yield of aerobic and anaerobic bottles. 30ml blood was distributed to one anaerobic and two aerobic bottles. Among 26,855 collections of ≥60 ml within 30min, 1379 (5.1%) were positive for a pathogen not requiring detection in more than one set to be considered a pathogen, with 72 additional distinct pathogens detected using two 30ml compared to two 20ml sets of one aerobic and one anaerobic bottle [increased yield, 7.9% (95%CI, 6.2-9.8%)]. For conditional pathogens requiring detection in at least two positive blood cultures for classification as a pathogen (i.e., otherwise classified as contaminants), there were 162 positive detections with two 30ml sets, of which 16 would not have been detected by two 20ml sets [increased yield, 11.0% (95%CI, 6.4-17.2%)]. Among 134 subjects who had three sets of 30ml each within a 30min interval, there was complete concordance between 60ml of blood drawn in the first two sets of 30ml and three 20ml sets (p=1.0). One aerobic plus anaerobic yielded more pathogens than two aerobic bottles for organisms requiring a single (p<0.001), and two (p=0.04) positive sets to be defined as a pathogen. In conclusion, we showed that collection of two aerobic and one anaerobic blood culture bottle per set results in improved yield compared to two bottles per set. We also confirmed that an anaerobic bottle should be included in blood culture sets. |
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Authors:
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Robin Patel; Emily A Vetter; W Scott Harmsen; Cathy D Schleck; Hind J Fadel; Franklin R Cockerill |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-5 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: - ISSN: 1098-660X ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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