| An optimized mouse model for transient ischemic attack. | |
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MedLine Citation:
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PMID: 20084015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transient ischemic attacks (TIAs) are brief neurological deficits ofcerebrovascular origin that are followed by complete clinical recovery. Although a plethora of animal models exist for ischemic stroke, a verified TIA model is lacking. We aimed to optimize such a model in mice, investigating the impact of varying durations (from 2.5 to 20 minutes) of intraluminal middle cerebral artery occlusion (MCAo). Three conditions were required to mimic clinical TIA reliably: 1) an objective demonstration of occlusion and reperfusion (assessed by laser Doppler flowmetry); 2) no permanent neurological deficit (assessed by sensorimotor neurological evaluation); and 3) no lesion at 24 hours after reperfusion (assessed by magnetic resonance imaging [MRI]). We observed high incidences of MRI lesions with MCAo durations of 15 minutes or longer. In contrast, no permanent neurological deficits or MRI lesions were observed in animals with MCAo below or equal to 10 minutes. Middle cerebral artery occlusion of 12.5 minutes rarely induced MRI lesions, but histopathologic evaluation using routine and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling staining revealed minute ischemic changes even after 2.5-minute MCAo. Abundance of necrotic and apoptotic changes gradually increased with the duration of ischemia. These results indicate that 10 minutes or shorter focal cerebral ischemia proves a suitable mouse TIA model; in addition, they indicate that MRI-negative microscopic ischemic damage may occur with even a few minutes of arterial occlusion. |
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Authors:
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Eric Pedrono; Aysan Durukan; Daniel Strbian; Ivan Marinkovic; Shashank Shekhar; Miia Pitkonen; Usama Abo-Ramadan; Turgut Tatlisumak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuropathology and experimental neurology Volume: 69 ISSN: 0022-3069 ISO Abbreviation: J. Neuropathol. Exp. Neurol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-03-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985192R Medline TA: J Neuropathol Exp Neurol Country: United States |
Other Details:
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Languages: eng Pagination: 188-95 Citation Subset: IM |
Affiliation:
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Experimental MRI Laboratory, Biomedicum Helsinki, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Brain / pathology, physiopathology Cerebral Infarction / diagnosis, etiology Disease Models, Animal* Follow-Up Studies In Situ Nick-End Labeling Infarction, Middle Cerebral Artery / complications* Ischemic Attack, Transient / complications, diagnosis, etiology*, physiopathology Laser-Doppler Flowmetry Magnetic Resonance Imaging Male Mice* Mice, Inbred Strains Necrosis Nervous System Diseases / etiology Time Factors |
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