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Optimization of xylanase production by Melanocarpus albomyces IIS68 in solid state fermentation using response surface methodology.
MedLine Citation:
PMID:  16233017     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Xylanase production by the thermophilic fungus, Melanocarpus albomyces IIS68, during solid state fermentation of wheat straw was studied and the effects of various variables were observed. Using the response surface methodology and the multivariant statistical approach, the optimum levels of the variables affecting xylanase production were determined. The optimum levels of the variables were 600-850 microm particle size, 43 h inoculum age, 1.37% Tween 80, 86% initial moisture content, 5.1% urea, 0.74% yeast extract and a harvest time of 96 h. Under these optimized conditions, xylanase activity of 7760 U/g initial dry substrate was obtained which was in very good agreement with the value predicted by the quadratic model (7890 U/g initial dry substrate).
Authors:
S Narang; V Sahai; V S Bisaria
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  91     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2001  
Date Detail:
Created Date:  2005-10-19     Completed Date:  2005-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  425-7     Citation Subset:  -    
Affiliation:
Department of Biochemical Engineering and Biotechnology, Indian Institute of technology, Delhi, Hauz Khas, New Delhi-110016, India.
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