Document Detail


Optimization and validation of a colorimetric assay for Tetrahymena sp. survival.
MedLine Citation:
PMID:  16300932     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A colorimetric assay based on the reduction of 3-(4,5-dimethyl-2-thiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide (MTT), for the quantification of Tetrahymena sp. survival is described. An increase in the concentration of Tetrahymena sp. cells from 0 to 1 x 10(6) cells/ml produced a linear (R(2)=0.9965) increase in the optical density (OD, 570-630 nm), and dead cells (pre-exposed to 250 mg/l formalin for 4 h) did not produce a background reading. Cells exposed to sublethal concentrations to formalin (100 mg/l or less for 4 h) recovered their growth. Using the MTT assay, we determined that Tetrahymena sp. is sensitive to formalin, chloramine-T, hydrogen peroxide, copper sulfate and NaCl. The sensitivity increased with increasing chemical concentrations and exposure time. Tetrahymena sp. was resistant to bromex and malachite green. The use of this assay in drug screening for the development of treatments for tetrahymenosis and as a bioassay to evaluate the toxicity of environmental toxicants is discussed.
Authors:
Dina Zilberg; Tamar Sinai
Publication Detail:
Type:  Journal Article; Validation Studies     Date:  2005-10-27
Journal Detail:
Title:  Research in microbiology     Volume:  157     ISSN:  0923-2508     ISO Abbreviation:  Res. Microbiol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-24     Completed Date:  2006-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907468     Medline TA:  Res Microbiol     Country:  France    
Other Details:
Languages:  eng     Pagination:  355-9     Citation Subset:  IM    
Affiliation:
Albert Katz Department of Dryland Biotechnologies, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus, 84990 Midreshet Ben Gurion, Israel. dzilberg@bgu.ac.il
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects
Cells, Cultured
Chloramines / toxicity
Colorimetry
Copper Sulfate / toxicity
Drug Evaluation, Preclinical
Environmental Pollutants / toxicity
Formaldehyde / toxicity
Hydrogen Peroxide / toxicity
Rosaniline Dyes / toxicity
Sodium Chloride / toxicity
Tetrahymena / cytology,  drug effects*
Tetrazolium Salts / chemistry
Thiazoles / chemistry
Tosyl Compounds / toxicity
Chemical
Reg. No./Substance:
0/Chloramines; 0/Environmental Pollutants; 0/Rosaniline Dyes; 0/Tetrazolium Salts; 0/Thiazoles; 0/Tosyl Compounds; 10309-95-2/malachite green; 127-65-1/chloramine-T; 298-93-1/thiazolyl blue; 50-00-0/Formaldehyde; 7647-14-5/Sodium Chloride; 7722-84-1/Hydrogen Peroxide; 7758-98-7/Copper Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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