Document Detail

Optimization and validation of capillary electrophoretic method for the analysis of amphenicols in poultry tissues.
MedLine Citation:
PMID:  18536172     Owner:  NLM     Status:  MEDLINE    
A simple, rapid capillary electrophoretic (CE) method for simultaneous analysis of three amphenicols (chloramphenicol, thiamphenicol and florfenicol) in poultry tissues has been developed and validated. The separation condition were: buffer solution composed with 25 mM sodium tetraborate decahydrate and 10 mM sodium dodecylsulfate, an uncoated fused-silica capillary (57 cm x 75 microm i.d.), voltage 20 kV, and temperature 22 degrees C. The method involved simple deproteinization by acetonitrile and SPE extraction procedure. The analytical method was validated according to the FDA bioanalytical method guidance. The method was linear (r > 0.999) at concentrations ranging from: 0.005 - 1 for chloramphenicol, 0.01 - 1 for thiamphenicol, and 0.025 - 5 microg/g for florfenicol. The precision values were less than 9.8 for intra- and 14.8% for inter-day variability, and accuracies ranged from 92.0 to 106.0% for analyzed amphenicols. The overall recoveries of all antibiotics from tissue samples were above 82.2%. Some new parameters were calculated as limit of decision (CCalpha) and detection capability (CCbeta). The CE method is simple and reliable for simultaneous determination of residues of chloramphenicol, thiamphenicol and florfenicol in muscle with a total run time of less than 7 min.
Piotr Kowalski; Alina Plenis; Ilona Oledzka
Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Acta poloniae pharmaceutica     Volume:  65     ISSN:  0001-6837     ISO Abbreviation:  Acta Pol Pharm     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-06-09     Completed Date:  2008-06-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985167R     Medline TA:  Acta Pol Pharm     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  45-50     Citation Subset:  IM    
Medical University of Gdańsk, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, 80-416 Gdańsk, Poland.
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MeSH Terms
Anti-Bacterial Agents / analysis*
Chloramphenicol / analysis
Drug Residues / analysis*
Electrophoresis, Capillary / methods*
Meat / analysis*
Reproducibility of Results
Thiamphenicol / analogs & derivatives,  analysis
Reg. No./Substance:
0/Anti-Bacterial Agents; 15318-45-3/Thiamphenicol; 56-75-7/Chloramphenicol; 73231-34-2/florfenicol

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