| Optimization and validation of an LC-fLD method for biotin in infant formula, infant cereals, cocoa-malt beverages, and clinical nutrition products. | |
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MedLine Citation:
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PMID: 21140662 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fast and simple chromatographic method to determine biotin in foods is presented. Biotin is extracted using papain (60 degrees C, 1 h). After pH adjustment and filtration, biotin is determined by LC with fluorescence detection using postcolumn reagent avidin-FITC (avidin labeled with fluorescein isothiocyanate). The method has been validated in a large range of products: milk- and soy-based infant formulas, cereals, cocoa-malt beverages, and clinical nutrition products. The method showed recovery rates of 98.1 +/- 5.7% (average +/- SD) in a large range of concentrations. Biotin concentrations determined in infant formula standard reference materials 1846 and 1849 were in agreement with reference values. RSD of repeatability (RSDr) varied from 2.0 to 4.5%, and intermediate reproducibility (RSD(iR)) from 5.8 to 9.4%. LOD and LOQ were 3.0 and 5.0 microg/100 g, respectively. The proposed method is suitable for routine analysis of biotin in fortified foods (infant formulas, infant cereals, cocoa-malt beverages, and clinical nutrition products). It can be used as a faster, more selective, and precise alternative to the classical microbiological determination, and is easily transferable among laboratories. |
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Authors:
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Esther Campos Giménez; Marie-Jose Trisconi; Tamara Kilinc; Pierre Andrieux |
Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: Journal of AOAC International Volume: 93 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-12-09 Completed Date: 2010-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 1494-502 Citation Subset: IM |
Affiliation:
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Nestlé Research Center, Vers-chez-les-blanc, CH-1000 Lausanne 26, Switzerland. esther.campos-gimenez@rdls.nestle.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Beverages
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analysis* Biotin / analysis* Cacao / chemistry* Cereals / chemistry* Chromatography, High Pressure Liquid / methods* Fluorescence Humans Infant Infant Formula / chemistry* Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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58-85-5/Biotin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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