| Optimization of rosmarinic acid production by Lavandula vera MM plant cell suspension in a laboratory bioreactor. | |
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MedLine Citation:
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PMID: 15801776 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The all-round effect of dissolved oxygen concentration, agitation speed, and temperature on the rosmarinic acid production by Lavandula vera MM cell suspension was studied in a 3-L laboratory bioreactor by means of the modified Simplex method. Polynomial regression models were elaborated for description of the process of rosmarinic acid production (Y) in the bioreactor as a consequence of the variation of the dissolved oxygen (X(1)) concentration between 10% and 50%; agitation (X(2)) between 100 and 400 rpm; and temperature (X(3)) between 22 and 30 degrees C. The optimization made it possible to establish the optimal conditions for the biosynthesis of rosmarinic acid by L. vera MM: dissolved oxygen (X(1)*), 50% of air saturation; agitation (X(2)*), 400 rpm; and temperature (X(3)*), 29.9 degrees C, where maximal yield (Y(max)) of 3489.4 mg/L of rosmarinic acid was achieved (2 times higher compared with the shake-flasks cultivation). |
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Authors:
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Atanas I Pavlov; Milen I Georgiev; Ivan N Panchev; Mladenka P Ilieva |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology progress Volume: 21 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2005 Mar-Apr |
Date Detail:
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Created Date: 2005-04-01 Completed Date: 2005-07-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 394-6 Citation Subset: IM |
Affiliation:
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Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bioreactors* Cinnamates / metabolism* Depsides Lavandula / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cinnamates; 0/Depsides; 537-15-5/rosmarinic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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