Document Detail

Optimization of rosmarinic acid production by Lavandula vera MM plant cell suspension in a laboratory bioreactor.
MedLine Citation:
PMID:  15801776     Owner:  NLM     Status:  MEDLINE    
The all-round effect of dissolved oxygen concentration, agitation speed, and temperature on the rosmarinic acid production by Lavandula vera MM cell suspension was studied in a 3-L laboratory bioreactor by means of the modified Simplex method. Polynomial regression models were elaborated for description of the process of rosmarinic acid production (Y) in the bioreactor as a consequence of the variation of the dissolved oxygen (X(1)) concentration between 10% and 50%; agitation (X(2)) between 100 and 400 rpm; and temperature (X(3)) between 22 and 30 degrees C. The optimization made it possible to establish the optimal conditions for the biosynthesis of rosmarinic acid by L. vera MM: dissolved oxygen (X(1)*), 50% of air saturation; agitation (X(2)*), 400 rpm; and temperature (X(3)*), 29.9 degrees C, where maximal yield (Y(max)) of 3489.4 mg/L of rosmarinic acid was achieved (2 times higher compared with the shake-flasks cultivation).
Atanas I Pavlov; Milen I Georgiev; Ivan N Panchev; Mladenka P Ilieva
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  21     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2005 Mar-Apr
Date Detail:
Created Date:  2005-04-01     Completed Date:  2005-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  394-6     Citation Subset:  IM    
Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria.
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MeSH Terms
Cinnamates / metabolism*
Lavandula / cytology,  metabolism*
Reg. No./Substance:
0/Cinnamates; 0/Depsides; 537-15-5/rosmarinic acid

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