Document Detail


Optimization of resolution in capillary zone electrophoresis: combined effect of applied voltage and buffer concentration.
MedLine Citation:
PMID:  1818085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expressions are formulated for the prediction of solute migration time and resolution as a function applied voltage and buffer concentration in capillary zone electrophoresis. The resolution equation assumes that solute diffusion is the only operative zone-broadening mechanism. A resolution surface in applied voltage and buffer concentration space is presented featuring isochrones that are used to predict the behavior of resolution under constant analysis time. In the resolution-voltage planes the resolution increases continuously with increasing voltage. At the high-voltage border, the resolution decreases continuously with increasing concentration, however, at the low-voltage border the resolution passes through a shallow maximum as the buffer concentration is increased. At constant analysis time, resolution is optimized by simultaneously increasing the voltage and the buffer concentration. In comparison, this theoretical approach, which predicts resolution from solute migration times only, gives values that are consistently about 40-50% higher than experimentally determined resolution.
Authors:
I Z Atamna; H J Issaq; G M Muschik; G M Janini
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of chromatography     Volume:  588     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-07-13     Completed Date:  1992-07-13     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  315-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates
Buffers
Capillary Action
Dansyl Compounds / chemistry
Electricity
Electrophoresis / methods*
Hexanones / chemistry
Leucine / analogs & derivatives,  chemistry
Mathematics
Methionine / analogs & derivatives,  chemistry
Grant Support
ID/Acronym/Agency:
N01-CO-74102/CO/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Acetates; 0/Buffers; 0/Dansyl Compounds; 0/Hexanones; 1100-22-7/dansylleucine; 17039-58-6/dansylmethionine; 77LAC84669/mesityl oxide; AE28F7PNPL/Methionine; GMW67QNF9C/Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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