Document Detail


Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems.
MedLine Citation:
PMID:  19183792     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mass spectrometry-based proteomics is a powerful analytical tool for investigating pathogens and their interactions within a host. The sensitivity of such analyses provides broad proteome characterization, but the sample-handling procedures must first be optimized to ensure compatibility with the technique and to maximize the dynamic range of detection. The decision-making process for determining optimal growth conditions, preparation methods, sample analysis methods, and data analysis techniques in our laboratory is discussed herein with consideration of the balance in sensitivity, specificity, and biomass losses during analysis of host-pathogen systems.
Authors:
Heather M Mottaz-Brewer; Angela D Norbeck; Joshua N Adkins; Nathan P Manes; Charles Ansong; Liang Shi; Yasuko Rikihisa; Takane Kikuchi; Scott W Wong; Ryan D Estep; Fred Heffron; Ljiljana Pasa-Tolic; Richard D Smith
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomolecular techniques : JBT     Volume:  19     ISSN:  1943-4731     ISO Abbreviation:  J Biomol Tech     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-04-02     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  100888641     Medline TA:  J Biomol Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  285-95     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anaplasma phagocytophilum / chemistry,  pathogenicity
Animals
Biotechnology
Chromatography, High Pressure Liquid
Ehrlichia chaffeensis / chemistry,  pathogenicity
HeLa Cells
Host-Pathogen Interactions*
Humans
Mass Spectrometry / methods,  statistics & numerical data
Monkeypox virus / chemistry,  physiology
Proteome / isolation & purification*
Proteomics / methods*,  statistics & numerical data
Salmonella / chemistry,  pathogenicity
Sensitivity and Specificity
Systems Biology / methods,  statistics & numerical data
Tandem Mass Spectrometry
Vaccinia virus / chemistry,  pathogenicity
Grant Support
ID/Acronym/Agency:
P41 RR018522/RR/NCRR NIH HHS; P41 RR018522-06/RR/NCRR NIH HHS; RR018522/RR/NCRR NIH HHS; Y1-AI-4894-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Proteome
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  DNA methylation signature analysis: how easy is it to perform?
Next Document:  Optimization of immobilized gallium (III) ion affinity chromatography for selective binding and reco...