Document Detail


Optimization of the production of thermostable endo-beta-1,4 mannanases from a newly isolated Aspergillus niger gr and Aspergillus flavus gr.
MedLine Citation:
PMID:  18597050     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this work was to establish optimal conditions for the maximum production of endo-beta-1,4 mannanases using cheaper sources. Eight thermotolerant fungal strains were isolated from garden soil and compost samples collected in and around the Gulbarga University campus, India. Two strains were selected based on their ability to produce considerable endo-beta-1,4 mannanases activity while growing in liquid medium at 37 degrees C with locust bean gum (LBG) as the only carbon source. They were identified as Aspergillus niger gr and Aspergillus flavus gr. The experiment to evaluate the effect of different carbon sources, nitrogen sources, temperatures and initial pH of the medium on maximal enzyme production was studied. Enzyme productivity was influenced by the type of polysaccharide used as the carbon source. Copra meal defatted with n-hexane showed to be a better substrate than LBG and guar gum for endo-beta-1,4 mannanases production by A. niger gr (40.011 U/ml), but for A. flavus gr (33.532 U/ml), the difference was not significant. Endo-beta-1,4 mannanases produced from A. niger gr and A. flavus gr have high optimum temperature (65 and 60 degrees C) and good thermostability in the absence of any stabilizers (maintaining 50% of residual activity for 8 and 6 h, respectively, at 60 degrees C) and are stable over in a wide pH range. These new strains offer an attractive alternative source of enzymes for the food and feed processing industries.
Authors:
Naganagouda V Kote; Aravind Goud G Patil; V H Mulimani
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-03
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  152     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2008-12-23     Completed Date:  2009-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  213-23     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Gulbarga University, Karnataka, India.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus flavus / classification,  isolation & purification*,  metabolism*
Aspergillus niger / classification,  isolation & purification*,  metabolism*
Enzyme Induction
Hydrogen-Ion Concentration
Mannosidases / biosynthesis*,  chemistry*,  metabolism
Nitrogen / chemistry,  pharmacology
Protein Stability
Temperature
Chemical
Reg. No./Substance:
7727-37-9/Nitrogen; EC 3.2.1.-/Mannosidases; EC 3.2.1.78/endo-1,4-beta-D-mannanase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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