Document Detail

Optimization of procedures for collecting and storing of CSF for studying the metabolome in ALS.
MedLine Citation:
PMID:  19412814     Owner:  NLM     Status:  MEDLINE    
There is a need for biomarkers for early diagnosis, development and evaluation of treatment efficacy in amyotrophic lateral sclerosis (ALS). We aimed to investigate if pre-analytical factors induce artefacts in metabolomic data of cerebrospinal fluid (CSF) from patients with ALS. CSF from 16 patients was studied using a statistical experimental design protocol with the following parameters: storage temperature (-80 degrees C/ - 20 degrees C), type of collection tube (polypropylene/polystyrene), and time delay from collecting to freezing (0, 10, 30, 90, 150 min). Gas chromatography-mass spectrometry was used to analyse CSF from 12 of the patients while CSF from one patient was analysed with nuclear magnetic resonance spectroscopy. The extent of CO(2) evaporization from CSF collected in tubes of different sizes at different temperatures and with/without lid were studied in three addtional patients. We found that alterations in storage temperature affect the metabolite composition of CSF more than any other studied pre-analytical parameter. CO(2) evaporization may induce artefacts in the metabolome by increasing the pH. In conclusion, minimization of evaluated artefacts can be obtained by collecting the CSF directly into tubes with tightly sealed lids in N(2)(l) and after freezing transfer of the tubes to -80 degrees C to minimize evaporation of CO(2).
Anna Wuolikainen; Mattias Hedenström; Thomas Moritz; Stefan L Marklund; Henrik Antti; Peter M Andersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Amyotrophic lateral sclerosis : official publication of the World Federation of Neurology Research Group on Motor Neuron Diseases     Volume:  10     ISSN:  1471-180X     ISO Abbreviation:  Amyotroph Lateral Scler     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-24     Completed Date:  2009-10-22     Revised Date:  2009-11-17    
Medline Journal Info:
Nlm Unique ID:  101283386     Medline TA:  Amyotroph Lateral Scler     Country:  England    
Other Details:
Languages:  eng     Pagination:  229-36     Citation Subset:  IM    
Department of Neurology, Umeå University Hospital, Umeå, Sweden.
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MeSH Terms
Amyotrophic Lateral Sclerosis* / cerebrospinal fluid,  metabolism,  physiopathology
Biological Markers / cerebrospinal fluid*
Carbon Dioxide / metabolism
Cerebrospinal Fluid / chemistry,  metabolism*
Gas Chromatography-Mass Spectrometry / methods
Hydrogen-Ion Concentration
Middle Aged
Nuclear Magnetic Resonance, Biomolecular
Reproducibility of Results
Specimen Handling / methods*
Reg. No./Substance:
0/Biological Markers; 124-38-9/Carbon Dioxide

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