Document Detail


Optimization of a preparative multimodal ion exchange step for purification of a potential malaria vaccine.
MedLine Citation:
PMID:  25271026     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In 2000 the implementation of quality by design (QbD) was introduced by the Food and Drug Administration (FDA) and described in the ICH Q8, Q9 and Q10 guidelines. Since that time, systematic optimization strategies for purification of biopharmaceuticals have gained a more important role in industrial process development. In this investigation, the optimization strategy was carried out by adopting design of experiments (DoE) in small scale experiments. A combination method comprising a desalting and a multimodal ion exchange step was used for the experimental runs via the chromatographic system ÄKTA™ avant. The multimodal resin Capto™ adhere was investigated as an alternative to conventional ion exchange and hydrophobic interaction resins for the intermediate purification of the potential malaria vaccine D1M1. The ligands, used in multimodal chromatography, interact with the target molecule in different ways. The multimodal functionality includes the binding of proteins in spite of the ionic strength of the loading material. The target protein binds at specific salt conditions and can be eluted by a step gradient decreasing the pH value and reducing the ionic strength. It is possible to achieve a maximized purity and recovery of the product because degradation products and other contaminants do not bind at specific salt concentrations at which the product still binds to the ligands.
Authors:
Jessica Paul; Sonja Jensen; Arthur Dukart; Gesine Cornelissen
Related Documents :
23257976 - Vitamin d-binding protein interacts with aβ and suppresses aβ-mediated pathology.
12186846 - Targeting mucosal sites by polymeric immunoglobulin receptor-directed peptides.
1973046 - 3,4-dihydroxyphenylethylamine, l-3,4-dihydroxyphenylalanine and 3,4,5-trihydroxyphenyla...
20169066 - Modulation of alpha-synuclein aggregation by dopamine analogs.
7988726 - Adding a positive charge at residue 46 of drosophila alcohol dehydrogenase increases co...
17034816 - A novel lysine-rich domain and gtp binding motifs regulate the nucleolar retention of h...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-19
Journal Detail:
Title:  Journal of chromatography. A     Volume:  -     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-10-1     Completed Date:  -     Revised Date:  2014-10-2    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier B.V. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Integrating HIV testing into cervical cancer screening in Tanzania: an analysis of routine service d...
Next Document:  [Considering body ethics in the healthcare profession].