| Optimization of perfusion decalcification for bones and joints in rats. | |
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MedLine Citation:
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PMID: 11066033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Decalcification of osseous tissues by perfusion of decalcifying solution into the vascular system has never been applied to the study of peripheral joints. To optimize perfusion methods, rats were decalcified by direct immersion or by one of two perfusion techniques: 1) systemic perfusion circulating the decalcifying solution from the ascending aorta; and 2) regional perfusion circulating the solution to the lower extremities from the abdominal aorta. The process of decalcification was monitored by serial radiographic examinations. After decalcification, bone and joint samples were stained for histochemistry and immunohistochemistry. With systemic perfusion, the decalcification time, dependent on weight, was markedly reduced compared to immersion. Regional perfusion decalcification was faster than all other methods studied. Microstructural preservation was comparable and immunostaining quality often improved. Applications of this work will improve the study of basic skeletal and articular problems. |
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Authors:
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G Trudel; M Seki; H K Uhthoff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Anatomical record Volume: 260 ISSN: 0003-276X ISO Abbreviation: Anat. Rec. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-20 Completed Date: 2000-11-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370540 Medline TA: Anat Rec Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 222-7 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
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Bone and Joint Research Laboratory, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone and Bones / cytology*, radiography Decalcification Technique* Immunoenzyme Techniques Joints / cytology* Male Perfusion / methods Rats Rats, Sprague-Dawley Time Factors Tissue Preservation / methods |
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